Cell Biology Antibodies 11

B9D1 Rabbit Polyclonal Antibody (CAB7973)

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SKU:
CAB7973
Product Type:
Antibody
Applications:
WB
Applications:
IHC
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
Antibody Type:
Polyclonal Antibody
Reactivity:
Human, Mouse, Rat
Synonyms:
B9, MKS9, EPPB9, MKSR1, JBTS27, MKSR-1, B9D1
Research Use:
Cell Biology
Frequently bought together:

Description

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B9D1 Antibody (CAB7973)

The B9D1 Antibody (CAB7973) is a high-quality antibody developed for reliable detection and analysis of target proteins. The antibody, produced in rabbits, exhibits high reactivity with human samples and is optimized for Western blot applications. By specifically binding to the B9D1 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it ideal for investigations in the fields of molecular biology and genetics.B9D1 is essential for ciliary function, playing a critical role in maintaining the structure and function of cilia, which are vital for cellular signaling and sensory processes.

This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:B9D1 Antibody
SKU:CAB7973
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MATA SPSV FLLM VNGQ VESA QFPE YDDL YCKY CFVY GQDW APTA GLEE GISQ ITSK SQDV RQAL VWNF PIDV TFKS TNPY GWPQ IVLS VYGP DVFG NDVV RGYG AVHV PFSP GRHK RTIP MFVP ESTS KL
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:2000
IHC-P1:50 - 1:200
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:B9, MKS9, EPPB9, MKSR1, JBTS27, MKSR-1, B9D1
Positive Sample:HeLa, Mouse testis, Mouse lung, Mouse ovary, Mouse kidney
Cellular Localization:Cytoplasm, Cilium Axoneme, Cilium Basal Body, Cytoskeleton.
Calculated MW:23kDa
Observed MW:23kDa

This gene encodes a B9 domain-containing protein, one of several that are involved in ciliogenesis. Alterations in expression of this gene have been found in a family with Meckel syndrome. Meckel syndrome has been associated with at least six different genes. This gene is located within the Smith-Magenis syndrome region on chromosome 17.

Purification MethodAffinity purification
Gene ID27077
RRIDAB_2768548
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using B9D1 Rabbit pAb (CAB7973) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human stomach using B9D1 Rabbit pAb (CAB7973) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Mouse lung using B9D1 Rabbit pAb (CAB7973) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunofluorescence analysis of L929 cells using B9D1 Rabbit pAb (CAB7973) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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