Name: Anti-B9D1 Antibody (CAB7973)
Brand: Cell Biology Antibodies 11
SKU: CAB7973
Availability: OutOfStock

Antibody Name:	B9D1 Rabbit Polyclonal Antibody
Antibody SKU:	CAB7973
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human B9D1 (NP_056496.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, Mouse testis, Mouse lung, Mouse ovary, Mouse kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human B9D1 (NP_056496.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MATA SPSV FLLM VNGQ VESA QFPE YDDL YCKY CFVY GQDW APTA GLEE GISQ ITSK SQDV RQAL VWNF PIDV TFKS TNPY GWPQ IVLS VYGP DVFG NDVV RGYG AVHV PFSP GRHK RTIP MFVP ESTS KL
Gene ID:	27077
Uniprot:	Q9UPM9
Cellular Location:	Cytoplasm, cilium axoneme, cilium basal body, cytoskeleton
Calculated MW:	16kDa/22kDa
Observed MW:	23kDa

Synonyms:	B9D1, B9, EPPB9, JBTS27, MKS9, MKSR1
Background:	This gene encodes a B9 domain-containing protein, one of several that are involved in ciliogenesis. Alterations in expression of this gene have been found in a family with Meckel syndrome. Meckel syndrome has been associated with at least six different genes. This gene is located within the Smith-Magenis syndrome region on chromosome 17.

UniProt Protein Function:	B9D1: Component of the tectonic-like complex, a complex localized at the transition zone of primary cilia and acting as a barrier that prevents diffusion of transmembrane proteins between the cilia and plasma membranes. Required for ciliogenesis and sonic hedgehog/SHH signaling. Defects in B9D1 are the cause of Meckel syndrome type 9 (MKS9). A disorder characterized by a combination of renal cysts and variably associated features including developmental anomalies of the central nervous system (typically encephalocele), hepatic ductal dysplasia and cysts, and polydactyly. Belongs to the B9D family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Chromosomal Location of Human Ortholog: 17p11.2 Cellular Component: centrosome; membrane; cytosol Molecular Function:hedgehog receptor activity Biological Process: smoothened signaling pathway; regulation of protein localization; camera-type eye development; vasculature development; in utero embryonic development; organelle organization and biogenesis; cilium biogenesis; embryonic digit morphogenesis Disease: Meckel Syndrome, Type 9
NCBI Summary:	This gene encodes a B9 domain-containing protein, one of several that are involved in ciliogenesis. Alterations in expression of this gene have been found in a family with Meckel syndrome. Meckel syndrome has been associated with at least six different genes. This gene is located within the Smith-Magenis syndrome region on chromosome 17. Three alternatively spliced transcript variants that encode different proteins have been described for this gene. [provided by RefSeq, Aug 2011]
UniProt Code:	Q9UPM9
NCBI GenInfo Identifier:	343478275
NCBI Gene ID:	27077
NCBI Accession:	NP_056496
UniProt Secondary Accession:	Q9UPM9,Q9BU22,
UniProt Related Accession:	Q9UPM9
Molecular Weight:	16,919 Da
NCBI Full Name:	B9 domain-containing protein 1 isoform a
NCBI Synonym Full Names:	B9 protein domain 1
NCBI Official Symbol:	B9D1
NCBI Official Synonym Symbols:	B9; MKS9; EPPB9; MKSR1
NCBI Protein Information:	B9 domain-containing protein 1; MKS1-related protein 1; endothelial precursor protein B9
UniProt Protein Name:	B9 domain-containing protein 1
UniProt Synonym Protein Names:	MKS1-related protein 1
Protein Family:	B9 domain-containing protein
UniProt Gene Name:	B9D1
UniProt Entry Name:	B9D1_HUMAN

	Western blot analysis of extracts of various cell lines, using B9D1 antibody (CAB7973) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human stomach using B9D1 antibody (CAB7973) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat kidney using B9D1 antibody (CAB7973) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse lung using B9D1 antibody (CAB7973) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of L929 cells using B9D1 Rabbit pAb (CAB7973) at dilution of 1:100. Blue: DAPI for nuclear staining.