Developmental Biology

Axl Rabbit Polyclonal Antibody (CAB17874)

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SKU:
CAB17874
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Developmental Biology
Synonyms:
ARK, UFO, JTK11, Tyro7, Axl
Research Use:
Developmental Biology
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Description

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Axl Antibody (CAB17874)

The Axl Antibody (CAB17874) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, specifically targets the AXL protein and has been validated for use in Western blot applications, providing reliable and accurate results.AXL is known for its role in cancer progression, metastasis, and drug resistance, making it a promising target for cancer therapy research. By using the AXL Polyclonal Antibody, researchers can detect and analyze AXL expression in different cell types, paving the way for a better understanding of its function in cancer biology.

This antibody is validated for use in WB, IHC-P, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human samples.

Product Name:Axl Antibody
SKU:CAB17874
Size:20μL, 100μL
Reactivity:Human
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:EESP FVGN PGNI TGAR GLTG TLRC QLQV QGEP PEVH WLRD GQIL ELAD STQT QVPL GEDE QDDW IVVS QLRI TSLQ LSDT GQYQ CLVF LGHQ TFVS QPGY VGLE GLPY FLEE PEDR TVAA NTPF NLSC QAQG PPEP VDLL WLQD AVPL ATAP GHGP QRSL HVPG LNKT SSFS CEAH NAKG VTTS RTAT ITVL PQQP RNLH LVSR QPTE LEVA WTPG LSGI YPLT HCTL QAVL SDDG MGIQ AGEP DPPE EPLT SQAS VPPH QLRL GSLH PHTP YHIR VACT SSQG PSSW THWL PVET PEGV PLGP PENI SATR NGSQ AFVH WQEP RAPL QGTL LGYR LAYQ GQDT PEVL MDIG LRQE VTLE LQGD GSVS NLTV CVAA YTAA GDGP WSLP VPLE AWRP GQAQ PVHQ LVKE PSTP AFSW P
Tested Applications:WB IHC-P IF/ICC IP ELISA
Recommended Dilution:
WB1:500 - 1:1000
IHC-P1:50 - 1:200
IF/ICC1:50 - 1:200
IP0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:ARK, UFO, JTK11, Tyro7, Axl
Positive Sample:HeLa, SW620, HepG2
Cellular Localization:Cell Membrane, Single-Pass Type I Membrane Protein.
Calculated MW:98kDa
Observed MW:140kDa

The protein encoded by this gene is a member of the Tyro3-Axl-Mer (TAM) receptor tyrosine kinase subfamily. The encoded protein possesses an extracellular domain which is composed of two immunoglobulin-like motifs at the N-terminal, followed by two fibronectin type-III motifs. It transduces signals from the extracellular matrix into the cytoplasm by binding to the vitamin K-dependent protein growth arrest-specific 6 (Gas6). This gene may be involved in several cellular functions including growth, migration, aggregation and anti-inflammation in multiple cell types. The encoded protein acts as a host cell receptor for multiple viruses, including Marburg, Ebola and Lassa viruses and is a candidate receptor for the SARS-CoV2 virus.

Purification MethodAffinity purification
Gene ID558
RRIDAB_2768533
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using Axl Rabbit pAb (CAB17874) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human lung cancer using Axl Rabbit pAb (CAB17874) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunofluorescence analysis of HeLa cells using Axl Rabbit pAb (CAB17874) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Product ImageImmunoprecipitation analysis of 300 μg extracts of HeLa cells using 3 μg Axl antibody (CAB17874). Western blot was performed from the immunoprecipitate using AXL antibody (CAB17874) at a dilution of 1:1000.
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