Cell Biology Antibodies 10

Anti-ATP2B2 Antibody (CAB6875)

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SKU:
CAB6875
Product Type:
Antibody
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-ATP2B2 Antibody
Antibody SKU:CAB6875
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-80 of human ATP2B2 (NP_001674.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Mouse, Rat
Positive Samples:Mouse ovary, Mouse lung, Mouse liver, Rat liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-80 of human ATP2B2 (NP_001674.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MGDM TNSD FYSK NQRN ESSH GGEF GCTM EELR SLME LRGT EAVV KIKE TYGD TEAI CRRL KTSP VEGL PGTA PDLE KRKQ
Gene ID:491
Uniprot:Q01814
Cellular Location:Cell junction, Cell membrane, Multi-pass membrane protein, synapse
Calculated MW:127-136kDa
Observed MW:137kDa
Synonyms:ATP2B2, PMCA2, PMCA2a, PMCA2i
Background:The protein encoded by this gene belongs to the family of P-type primary ion transport ATPases characterized by the formation of an aspartyl phosphate intermediate during the reaction cycle. These enzymes remove bivalent calcium ions from eukaryotic cells against very large concentration gradients and play a critical role in intracellular calcium homeostasis. The mammalian plasma membrane calcium ATPase isoforms are encoded by at least four separate genes and the diversity of these enzymes is further increased by alternative splicing of transcripts. The expression of different isoforms and splice variants is regulated in a developmental, tissue- and cell type-specific manner, suggesting that these pumps are functionally adapted to the physiological needs of particular cells and tissues. This gene encodes the plasma membrane calcium ATPase isoform 2. Alternatively spliced transcript variants encoding different isoforms have been identified.
UniProt Protein Function:ATP2B2: This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell. Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIB subfamily. 8 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Membrane protein, multi-pass; EC 3.6.3.8; Membrane protein, integral; Transporter, ion channel; Endoplasmic reticulum; Hydrolase; Cell development/differentiation; Transporter

Chromosomal Location of Human Ortholog: 3p25.3

Cellular Component: cell soma; endoplasmic reticulum; cytoplasm; apical plasma membrane; integral to membrane; plasma membrane; synapse; cell junction; cilium

Molecular Function:protein C-terminus binding; calmodulin binding; protein binding; calcium-transporting ATPase activity; metal ion binding; calcium ion binding; calcium-dependent ATPase activity; ATP binding; PDZ domain binding

Biological Process: lactation; cytosolic calcium ion homeostasis; organelle organization and biogenesis; otolith mineralization; regulation of synaptic plasticity; locomotory behavior; detection of mechanical stimulus involved in sensory perception of sound; cerebellar granule cell differentiation; neuron differentiation; sensory perception of sound; transport; calcium ion transport; cerebellar Purkinje cell differentiation; synapse organization and biogenesis; auditory receptor cell stereocilium organization and biogenesis; blood coagulation; locomotion; neuromuscular process controlling balance; positive regulation of calcium ion transport; transmembrane transport; cGMP metabolic process; regulation of cell size; serotonin metabolic process

Disease: Deafness, Autosomal Recessive 12

NCBI Summary:The protein encoded by this gene belongs to the family of P-type primary ion transport ATPases characterized by the formation of an aspartyl phosphate intermediate during the reaction cycle. These enzymes remove bivalent calcium ions from eukaryotic cells against very large concentration gradients and play a critical role in intracellular calcium homeostasis. The mammalian plasma membrane calcium ATPase isoforms are encoded by at least four separate genes and the diversity of these enzymes is further increased by alternative splicing of transcripts. The expression of different isoforms and splice variants is regulated in a developmental, tissue- and cell type-specific manner, suggesting that these pumps are functionally adapted to the physiological needs of particular cells and tissues. This gene encodes the plasma membrane calcium ATPase isoform 2. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
UniProt Code:Q01814
NCBI GenInfo Identifier:14286115
NCBI Gene ID:491
NCBI Accession:Q01814.2
UniProt Related Accession:Q01814
Molecular Weight:
NCBI Full Name:Plasma membrane calcium-transporting ATPase 2
NCBI Synonym Full Names:ATPase plasma membrane Ca2+ transporting 2
NCBI Official Symbol:ATP2B2  
NCBI Official Synonym Symbols:PMCA2; PMCA2a; PMCA2i  
NCBI Protein Information:plasma membrane calcium-transporting ATPase 2
UniProt Protein Name:Plasma membrane calcium-transporting ATPase 2
UniProt Synonym Protein Names:Plasma membrane calcium ATPase isoform 2; Plasma membrane calcium pump isoform 2
UniProt Gene Name:ATP2B2  
UniProt Entry Name:AT2B2_HUMAN
Anti-ATP2B2 Antibody (CAB6875)Western blot analysis of extracts of various cell lines, using ATP2B2 antibody (CAB6875) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
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