Autophagy Antibodies

ATG9B Rabbit Polyclonal Antibody (CAB7406)

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SKU:
CAB7406
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Autophagy
Reactivity:
Human, Mouse, Rat
Synonyms:
SONE, APG9L2, NOS3AS, ATG9B
Research Use:
Autophagy
Frequently bought together:

Description

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ATG9B Antibody (CAB7406)

The ATG9B Antibody (CAB7406) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, specifically targets the ATG9B protein, a key player in the autophagy pathway.ATG9B is involved in the formation and maintenance of autophagosomes, which are responsible for degrading and recycling cellular components. Dysfunction in autophagy has been linked to various diseases, including neurodegenerative disorders and cancer. By targeting ATG9B with this antibody, researchers can visualize and analyze the protein's localization and expression levels in different cell types.

This antibody is validated for use in WB, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:ATG9B Antibody
SKU:CAB7406
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:HPLW RPPG HSSK FLGH LWGR VQQD AAAW GATS ARGP STPG VLSN CTSP LPEA FLAN LFVH PLLP PRDL SPTA PCPA AATA SLLA SISR IAQD PSSV SPGG TGGQ KLAQ LPEL ASAE MSLH VIYL HQLH QQQQ QQEP WGEA AASI LSRP CSSP SQPP SPDE EKPS WSSD GSSP ASSP RQQW GTQK ARNL FPGG FQVT TDTQ KEPD RASC TD
Tested Applications:WB ELISA IF-P
Recommended Dilution:
WB1:500 - 1:1000
IF-P1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:SONE, APG9L2, NOS3AS, ATG9B
Positive Sample:HUH-7, Mouse brain, Rat stomach
Cellular Localization:Cytoplasmic Vesicle, Multi-Pass Membrane Protein, Autophagosome Membrane.
Calculated MW:101kDa
Observed MW:100kDa

This gene functions in the regulation of autophagy, a lysosomal degradation pathway. This gene also functions as an antisense transcript in the posttranscriptional regulation of the endothelial nitric oxide synthase 3 gene, which has 3' overlap with this gene on the opposite strand. Mutations in this gene and disruption of the autophagy process have been associated with multiple cancers. Alternative splicing results in multiple transcript variants.

Purification MethodAffinity purification
Gene ID285973
RRIDAB_2767937
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of lysates from HUH-7 cells, using ATG9B Rabbit pAb (CAB7406) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Product ImageWestern blot analysis of various lysates using ATG9B Rabbit pAb (CAB7406) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
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