Autophagy Antibodies

ATG4A Rabbit Polyclonal Antibody (CAB2598)

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SKU:
CAB2598
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Autophagy
Antibody Type:
Polyclonal Antibody
Reactivity:
Human, Mouse, Rat
Synonyms:
APG4A, AUTL2, HsAPG4A, ATG4A
Research Use:
Autophagy
Frequently bought together:

Description

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ATG4A Antibody (CAB2598)

The ATG4A Antibody (CAB2598) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, demonstrates high specificity and reactivity towards human ATG4A, making it suitable for Western blot applications. By binding specifically to the ATG4A protein, researchers can accurately detect and analyze its expression in a variety of cell types, offering insights into autophagy mechanisms and potential therapeutic targets.

This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:ATG4A Antibody
SKU:CAB2598
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:SKGT SAYC SAWK PLLL IVPL RLGI NQIN PVYV DAFK ECFK MPQS LGAL GGKP NNAY YFIG FLGD ELIF LDPH TTQT FVDT EENG TVND QTFH CLQS PQRM NILN LDPS VALG FFCK EEKD FDNW CSLV QKEI LKEN LRMF ELVQ KHPS HWPP FVPP AKPE VTTT GAEF IDST EQLE EFDL EEDF EILS V
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:
WB1:100 - 1:500
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:APG4A, AUTL2, HsAPG4A, ATG4A
Positive Sample:HeLa, Mouse liver, Rat lung
Cellular Localization:Cytoplasm.
Calculated MW:45kDa
Observed MW:48kDa

Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases.

Purification MethodAffinity purification
Gene ID115201
RRIDAB_2764483
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using ATG4A Rabbit pAb (CAB2598) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Product ImageImmunofluorescence analysis of NIH/3T3 cells using ATG4A Rabbit pAb (CAB2598) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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