Name: Anti-ATG4A Antibody (CAB2598)
Brand: Autophagy Antibodies
SKU: CAB2598
Availability: OutOfStock

Antibody Name:	Anti-ATG4A Antibody
Antibody SKU:	CAB2598
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 289-398 of human ATG4A (NP_443168.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse
Positive Samples:	HepG2, Jurkat, 22Rv1, K-562, BT-474, Mouse skeletal muscle

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 289-398 of human ATG4A (NP_443168.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	TEEN GTVN DQTF HCLQ SPQR MNIL NLDP SVAL GFFC KEEK DFDN WCSL VQKE ILKE NLRM FELV QKHP SHWP PFVP PAKP EVTT TGAE FIDS TEQL EEFD LEED FEIL SV
Gene ID:	115201
Uniprot:	Q8WYN0
Cellular Location:	Cytoplasm
Calculated MW:	36kDa/38kDa/42kDa/45kDa
Observed MW:	45kDa

Synonyms:

ATG4A, APG4A, AUTL2

Background:

Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases.

UniProt Protein Function:	ATG4A: Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP. Widely expressed, at a low level, and the highest expression is observed in skeletal muscle and brain. Also detected in fetal liver. Inhibited by N-ethylmaleimide. Belongs to the peptidase C54 family. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Microtubule-binding; Autophagy; EC 3.4.22.-; Protease Chromosomal Location of Human Ortholog: Xq22.3 Cellular Component: cytoplasm; cytosol Molecular Function:cysteine-type endopeptidase activity; cysteine-type peptidase activity Biological Process: protein delipidation; mitochondrion degradation; protein processing; C-terminal protein lipidation; protein targeting to membrane; proteolysis; cellular response to nitrogen starvation; autophagic vacuole formation
NCBI Summary:	Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Transcript variants that encode distinct isoforms have been identified. [provided by RefSeq, Jul 2008]
UniProt Code:	Q8WYN0
NCBI GenInfo Identifier:	61211859
NCBI Gene ID:	115201
NCBI Accession:	Q8WYN0.1
UniProt Secondary Accession:	Q8WYN0,O95534, Q5JYY9, Q5JYZ0, Q86VE5, Q96KQ0, Q96KQ1 A6NCH2, B2RAZ7, D3DUY0,
UniProt Related Accession:	Q8WYN0
Molecular Weight:	398
NCBI Full Name:	Cysteine protease ATG4A
NCBI Synonym Full Names:	autophagy related 4A, cysteine peptidase
NCBI Official Symbol:	ATG4A
NCBI Official Synonym Symbols:	APG4A; AUTL2
NCBI Protein Information:	cysteine protease ATG4A; hAPG4A; autophagin 2; autophagin-2; APG4 autophagy 4 homolog A; AUT-like 2 cysteine endopeptidase; ATG4 autophagy related 4 homolog A; AUT-like 2, cysteine endopeptidase; autophagy-related protein 4 homolog A; autophagy-related cysteine endopeptidase 2
UniProt Protein Name:	Cysteine protease ATG4A
UniProt Synonym Protein Names:	AUT-like 2 cysteine endopeptidase; Autophagin-2; Autophagy-related cysteine endopeptidase 2; Autophagy-related protein 4 homolog A; hAPG4A
Protein Family:	Cysteine protease
UniProt Gene Name:	ATG4A
UniProt Entry Name:	ATG4A_HUMAN

Western blot analysis of extracts of various cell lines, using ATG4A antibody (CAB2598) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

Antibodies

Anti-ATG4A Antibody (CAB19844)[KO Validated]

ATG4A Antibody (PACO03399)

ATG4A Antibody (PACO07468)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)