Epigenetics & Nuclear Signaling Antibodies 1

Anti-ATF6 Antibody (CAB12570)

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SKU:
CAB12570
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-ATF6 Antibody
Antibody SKU:CAB12570
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 570 to the C-terminus of human ATF6 (NP_031374.2).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:K-562, Mouse brain
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 570 to the C-terminus of human ATF6 (NP_031374.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:SFRR DHLL LPAT THNK TTRP KMSI VLPA ININ ENVI NGQD YEVM MQID CQVM DTRI LHIK SSSV PPYL RDQQ RNQT NTFF GSPP AATE ATHV VSTI PESL Q
Gene ID:22926
Uniprot:P18850
Cellular Location:Endoplasmic reticulum membrane, Nucleus, Single-pass type II membrane protein
Calculated MW:74kDa
Observed MW:92kDa
Synonyms:ATF6, ACHM7, ATF6A
Background:This gene encodes a transcription factor that activates target genes for the unfolded protein response (UPR) during endoplasmic reticulum (ER) stress. Although it is a transcription factor, this protein is unusual in that it is synthesized as a transmembrane protein that is embedded in the ER. It functions as an ER stress sensor/transducer, and following ER stress-induced proteolysis, it functions as a nuclear transcription factor via a cis-acting ER stress response element (ERSE) that is present in the promoters of genes encoding ER chaperones. This protein has been identified as a survival factor for quiescent but not proliferative squamous carcinoma cells. There have been conflicting reports about the association of polymorphisms in this gene with diabetes in different populations, but another polymorphism has been associated with increased plasma cholesterol levels. This gene is also thought to be a potential therapeutic target for cystic fibrosis.
UniProt Protein Function:ATF6A: Transcription factor that acts during endoplasmic reticulum stress by activating unfolded protein response target genes. Binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N(9)-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. Could also be involved in activation of transcription by the serum response factor. Belongs to the bZIP family. ATF subfamily.
UniProt Protein Details:

Protein type:Transcription factor; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q23.3

Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane; Golgi apparatus; Golgi membrane; integral to endoplasmic reticulum membrane; membrane; nuclear envelope; nucleoplasm; nucleus

Molecular Function:identical protein binding; protein binding; protein heterodimerization activity; transcription coactivator activity; transcription factor activity

Biological Process: eye development; positive regulation of transcription of target genes involved in unfolded protein response; protein folding; regulation of transcription from RNA polymerase II promoter; response to stress; signal transduction; unfolded protein response; visual perception

Disease: Achromatopsia 7

NCBI Summary:This gene encodes a transcription factor that activates target genes for the unfolded protein response (UPR) during endoplasmic reticulum (ER) stress. Although it is a transcription factor, this protein is unusual in that it is synthesized as a transmembrane protein that is embedded in the ER. It functions as an ER stress sensor/transducer, and following ER stress-induced proteolysis, it functions as a nuclear transcription factor via a cis-acting ER stress response element (ERSE) that is present in the promoters of genes encoding ER chaperones. This protein has been identified as a survival factor for quiescent but not proliferative squamous carcinoma cells. There have been conflicting reports about the association of polymorphisms in this gene with diabetes in different populations, but another polymorphism has been associated with increased plasma cholesterol levels. This gene is also thought to be a potential therapeutic target for cystic fibrosis. [provided by RefSeq, Aug 2011]
UniProt Code:P18850
NCBI GenInfo Identifier:66774203
NCBI Gene ID:22926
NCBI Accession:P18850.3
UniProt Secondary Accession:P18850,O15139, Q5VW62, Q6IPB5, Q9UEC9,
UniProt Related Accession:P18850
Molecular Weight:74,585 Da
NCBI Full Name:Cyclic AMP-dependent transcription factor ATF-6 alpha
NCBI Synonym Full Names:activating transcription factor 6
NCBI Official Symbol:ATF6  
NCBI Official Synonym Symbols:ACHM7; ATF6A  
NCBI Protein Information:cyclic AMP-dependent transcription factor ATF-6 alpha
UniProt Protein Name:Cyclic AMP-dependent transcription factor ATF-6 alpha
UniProt Synonym Protein Names:Activating transcription factor 6 alpha; ATF6-alpha
Protein Family:Cyclic AMP-dependent transcription factor
UniProt Gene Name:ATF6  
UniProt Entry Name:ATF6A_HUMAN
Anti-ATF6 Antibody (CAB12570)Western blot analysis of extracts of various cell lines, using ATF6 antibody (CAB12570) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
Anti-ATF6 Antibody (CAB12570)Immunohistochemistry of paraffin-embedded human esophagus using ATF6 antibody (CAB12570) (40x lens).
Anti-ATF6 Antibody (CAB12570)Immunohistochemistry of paraffin-embedded rat brain using ATF6 antibody (CAB12570) (40x lens).
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