Cell Biology Antibodies 12
Anti-ATAD3B Antibody (CAB8268)
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- SKU:
- CAB8268
- Product Type:
- Antibody
- Applications:
- WB
- Applications:
- IHC
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | ATAD3B Rabbit Polyclonal Antibody |
| Antibody SKU: | CAB8268 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human ATAD3B (NP_114127.3). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | NIH/3T3, Mouse heart, Mouse spleen, Mouse brain, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human ATAD3B (NP_114127.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MSWL FGVN KGPK GEGA GPPP PLPP AQPG AEGG GDRG LGDR PAPK DKWS NFDP TGLE RAAK AARE LEHS RYAK EALN LAQM QEQT LQLE QQSK LKEY EAAV EQLK SEQI RAQA EERR KTLS EETR QHQA RAQY QDKL ARQR YEDQ LKQQ QLLN EENL RKQE ESVQ KQEA MRRA TVER EMEL RHKN EMLR VETE ARAR AKAE RENA DIIR EQIR LKAS EHRQ TVLE SIRT AGTL FGEG FRAF |
| Gene ID: | 83858 |
| Uniprot: | Q5T9A4 |
| Cellular Location: | Mitochondrion inner membrane, Peripheral membrane protein |
| Calculated MW: | 19kDa/67kDa/72kDa |
| Observed MW: | 68kDa |
| Synonyms: | ATAD3B, AAA-TOB3, TOB3, ATPase family |
| Background: | The protein encoded by this gene is localized to the mitochondrial inner membrane, where it can bind to a highly-related protein, ATAD3A. ATAD3A appears to interact with matrix nucleoid complexes, and the encoded protein negatively regulates that interaction. This gene is expressed almost exclusively in pluripotent embryonic stem cells and some cancer cells. Two transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | ATAD3B: May play a role in a mitochondrial network organization typical for stem cells, characterized by reduced mitochondrial metabolism, low mtDNA copies and fragmentated mitochondrial network. may act by suppressing ATAD3A function, interfering with ATAD3A interaction with matrix nucleoid complexes. Belongs to the AAA ATPase family. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Mitochondrial Chromosomal Location of Human Ortholog: 1p36.33 Cellular Component: mitochondrial inner membrane Molecular Function:ATP binding |
| NCBI Summary: | ATAD3A (MIM 612316) and ATAD3B are mitochondrial membrane proteins that contribute to the stabilization of large mitochondrial DNA (mtDNA)-protein complexes called nucleoids (He et al., 2007 [PubMed 17210950]).[supplied by OMIM, Sep 2008] |
| UniProt Code: | Q5T9A4 |
| NCBI GenInfo Identifier: | 75677353 |
| NCBI Gene ID: | 83858 |
| NCBI Accession: | NP_114127.3 |
| UniProt Secondary Accession: | Q5T9A4,Q6ZRB5, Q9BUK4, Q9ULE7, A8K3H1, |
| UniProt Related Accession: | Q5T9A4 |
| Molecular Weight: | 67,608 Da |
| NCBI Full Name: | ATPase family AAA domain-containing protein 3B |
| NCBI Synonym Full Names: | ATPase family, AAA domain containing 3B |
| NCBI Official Symbol: | ATAD3B |
| NCBI Official Synonym Symbols: | TOB3; AAA-TOB3 |
| NCBI Protein Information: | ATPase family AAA domain-containing protein 3B; AAA-ATPase TOB3 |
| UniProt Protein Name: | ATPase family AAA domain-containing protein 3B |
| UniProt Synonym Protein Names: | AAA-TOB3 |
| Protein Family: | ATPase family AAA domain-containing protein |
| UniProt Gene Name: | ATAD3B |
| UniProt Entry Name: | ATD3B_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ATAD3B antibody (CAB8268) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
 | Immunohistochemistry of paraffin-embedded mouse heart using ATAD3B antibody (CAB8268) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human liver damage using ATAD3B antibody (CAB8268) at dilution of 1:100 (40x lens). |
 | Confocal immunofluorescence analysis of U2OS cells using Anti-ATAD3B Polyclonal Antibody (CAB8268) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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