Cell Biology Antibodies 11

ASPSCR1 Rabbit Polyclonal Antibody (CAB7481)

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SKU:
CAB7481
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Reactivity:
Human, Mouse, Rat
Synonyms:
TUG, ASPL, ASPS, RCC17, UBXD9, UBXN9, ASPCR1, TUG/ASPSCR1
Research Use:
Cell Biology
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Description

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ASPSCR1 Antibody (CAB7481)

The ASPSCR1 Antibody (CAB7481) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly reactive with human samples and has been validated for use in various applications, including Western blot and immunohistochemistry.ASPSCR1, also known as TFE3 Fusion Partner, is a fusion gene that plays a critical role in the pathogenesis of alveolar soft part sarcoma, a rare soft tissue tumor. Understanding the function and expression of ASPSCR1 is crucial for unraveling the mechanisms underlying this disease and developing targeted therapies.

This antibody is validated for use in WB, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:ASPSCR1 Antibody
SKU:CAB7481
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:KSKS GQDP QQEQ EQER ERDP QQEQ ERER PVDR EPVD REPV VCHP DLEE RLQA WPAE LPDE FFEL TVDD VRRR LAQL KSER KRLE EAPL VTKA FREA QIKE KLER YPKV ALRV LFPD RYVL QGFF RPSE TVGD LRDF VRSH LGNP ELSF YLFI TPPK TVLD DHTQ TLFQ ANLF PAAL VHLG AEEP AGVY LEPG LLEH AISP SAAD VLVA RYMS RAAG SPSP LPAP DPAP KSEP AAEE GALV PPEP IPGT AQPV KRSL GKVP KWLK LPAS KR
Tested Applications:WB IF/ICC IP ELISA
Recommended Dilution:
WB1:500 - 1:2000
IF/ICC1:50 - 1:200
IP0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:TUG, ASPL, ASPS, RCC17, UBXD9, UBXN9, ASPCR1, TUG/ASPSCR1
Positive Sample:293T, SW480, A-549, BT-474, Mouse liver, Mouse testis
Cellular Localization:Cytoplasm, Endomembrane System, Endoplasmic Reticulum-Golgi Intermediate Compartment Membrane, Nucleus, Peripheral Membrane Protein.
Calculated MW:60kDa
Observed MW:80kDa

The protein encoded by this gene contains a UBX domain and interacts with glucose transporter type 4 (GLUT4). This protein is a tether, which sequesters the GLUT4 in intracellular vesicles in muscle and fat cells in the absence of insulin, and redistributes the GLUT4 to the plasma membrane within minutes of insulin stimulation. Translocation t(X;17)(p11;q25) of this gene with transcription factor TFE3 gene results in a ASPSCR1-TFE3 fusion protein in alveolar soft part sarcoma and in renal cell carcinomas. Multiple alternatively spliced transcript variants have been found.

Purification MethodAffinity purification
Gene ID79058
RRIDAB_2768012
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using TUG/ASPSCR1 Rabbit pAb (CAB7481) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Product ImageImmunofluorescence analysis of HeLa cells using TUG/ASPSCR1 Rabbit pAb (CAB7481) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Product ImageImmunofluorescence analysis of PC-12 cells using TUG/ASPSCR1 Rabbit pAb (CAB7481) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Product ImageImmunoprecipitation analysis of 200 μg extracts of A-549 cells, using 3 μg TUG/ASPSCR1 antibody (CAB7481). Western blot was performed from the immunoprecipitate using TUG/ASPSCR1 antibody (CAB7481) at a dilution of 1:1000.
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