The ApoM Monoclonal Antibody (CAB5144) is a high-quality antibody developed for reliable detection and analysis of target proteins. APOM is a critical component in lipid metabolism, specifically in the transport and metabolism of lipids such as cholesterol and sphingosine-1-phosphate. The antibody, generated in rabbits, is highly reactive with human samples and has been validated for use in various applications, including Western blot and immunohistochemistry.APOM is known to play a key role in maintaining lipid homeostasis and has been implicated in various metabolic disorders, including cardiovascular diseases and diabetes.
This antibody is validated for use in IF/ICC, ELISA, IF-P applications and has demonstrated reactivity against Human samples.
Product Name:
ApoM Monoclonal Antibody
SKU:
CAB5144
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC1210
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
G3a, NG20, apo-M, HSPC336, ApoM
Cellular Localization:
Secreted.
Calculated MW:
21kDa
Observed MW:
Refertofigures
The protein encoded by this gene is an apolipoprotein and member of the lipocalin protein family. It is found associated with high density lipoproteins and to a lesser extent with low density lipoproteins and triglyceride-rich lipoproteins. The encoded protein is secreted through the plasma membrane but remains membrane-bound, where it is involved in lipid transport. Alternate splicing results in both coding and non-coding variants of this gene.
Purification Method
Affinity purification
Gene ID
55937
RRID
AB_2863465
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Confocal imaging of Hep G2 cells using ApoM Rabbit mAb (CAB5144, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (CABS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (CABS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
Confocal imaging of paraffin-embedded Human liver cancer tissue using ApoM Rabbit mAb (CAB5144, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (CABS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
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