Cell Biology Antibodies 4

APEH Rabbit Polyclonal Antibody (CAB13467)

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SKU:
CAB13467
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Reactivity:
Human, Mouse, Rat
Synonyms:
APH, OPH, AARE, ACPH, D3S48E, D3F15S2, DNF15S2, APEH
Research Use:
Cell Biology
Frequently bought together:

Description

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APEH Antibody (CAB13467)

The APEH Antibody (CAB13467) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high specificity and sensitivity for detecting APEH in human samples, making it ideal for use in Western blot applications.APEH, also known as acylpeptide hydrolase, plays a pivotal role in maintaining cellular homeostasis by degrading small peptides and proteins. Dysregulation of APEH activity has been implicated in various diseases, including cancer and neurodegenerative disorders.

This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:APEH Antibody
SKU:CAB13467
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MERQ VLLS EPEE AAAL YRGL SRQP ALSA ACLG PEVT TQYG GQYR TVHT EWTQ RDLE RMEN IRFC RQYL VFHD GDSV VFAG PAGN SVET RGEL LSRE SPSG TMKA VLRK AGGT GPGE EKQF LEVW EKNR KLKS FNLS ALEK HGPV YEDD CFGC LSWS HSET HLLY VAEK KRPK AESF FQTK ALDV SASD DEIA RLKK PDQA IKGD QFVF YEDW GENM VSKS IPVL CVLD VESG NISV LEGV PENV SPGQ AFWA PGDA GVVF
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:2000
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:APH, OPH, AARE, ACPH, D3S48E, D3F15S2, DNF15S2, APEH
Positive Sample:SW480, Jurkat, Mouse liver, Mouse kidney, Mouse small intestine, Rat bone marrow
Cellular Localization:Cytoplasm.
Calculated MW:81kDa
Observed MW:80-108kDa

This gene encodes the enzyme acylpeptide hydrolase, which catalyzes the hydrolysis of the terminal acetylated amino acid preferentially from small acetylated peptides. The acetyl amino acid formed by this hydrolase is further processed to acetate and a free amino acid by an aminoacylase. This gene is located within the same region of chromosome 3 (3p21) as the aminoacylase gene, and deletions at this locus are also associated with a decrease in aminoacylase activity. The acylpeptide hydrolase is a homotetrameric protein of 300 kDa with each subunit consisting of 732 amino acid residues. It can play an important role in destroying oxidatively damaged proteins in living cells. Deletions of this gene locus are found in various types of carcinomas, including small cell lung carcinoma and renal cell carcinoma.

Purification MethodAffinity purification
Gene ID327
RRIDAB_2760328
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using APEH Rabbit pAb (CAB13467) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Product ImageImmunofluorescence analysis of HeLa cells using APEH Rabbit pAb (CAB13467). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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