Cell Biology Antibodies 10

AP2A1 Rabbit Polyclonal Antibody (CAB6863)

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SKU:
CAB6863
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Reactivity:
Human, Mouse, Rat
Synonyms:
ADTAA, CLAPA1, AP2-ALPHA, AP2A1
Research Use:
Cell Biology
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Description

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AP2A1 Antibody (CAB6863)

The AP2A1 Antibody (CAB6863) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the AP2A1 protein, this antibody enables the detection and analysis of AP2A1 in a variety of cell types, making it ideal for studies in cell biology and molecular biology.

This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:AP2A1 Antibody
SKU:CAB6863
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MPAV SKGD GMRG LAVF ISDI RNCK SKEA EIKR INKE LANI RSKF KGDK ALDG YSKK KYVC KLLF IFLL GHDI DFGH MEAV NLLS SNKY TEKQ IGYL FISV LVNS NSEL IRLI NNAI KNDL ASRN PTFM CLAL HCIA NVGS REMG EAFA ADIP RILV AGDS MDSV KQSA ALCL LRLY KASP DLVP MGEW TARV VHLL NDQH MGVV TAAV SLIT CLCK KNPD DFKT CVSL AVSR LSRI VSSA STDL QDYT YYFV PAPW LSVK
Tested Applications:WB ELISA
Recommended Dilution:
WB1:500 - 1:2000
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:ADTAA, CLAPA1, AP2-ALPHA, AP2A1
Positive Sample:THP-1, HeLa, Rat liver, Rat lung
Cellular Localization:Cell Membrane, Cytoplasmic Side, Membrane, Peripheral Membrane Protein, Coated Pit.
Calculated MW:108kDa
Observed MW:108kDa

This gene encodes the alpha 1 adaptin subunit of the adaptor protein 2 (AP-2) complex found in clathrin coated vesicles. The AP-2 complex is a heterotetramer consisting of two large adaptins (alpha or beta), a medium adaptin (mu), and a small adaptin (sigma). The complex is part of the protein coat on the cytoplasmic face of coated vesicles which links clathrin to receptors in vesicles. Alternative splicing of this gene results in two transcript variants encoding two different isoforms. A third transcript variant has been described, but its full length nature has not been determined.

Purification MethodAffinity purification
Gene ID160
RRIDAB_2767424
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using AP2A1 Rabbit pAb (CAB6863) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
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