Cell Biology Antibodies 11

ALKBH8 Rabbit Polyclonal Antibody (CAB7142)

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SKU:
CAB7142
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Reactivity:
Human, Mouse, Rat
Synonyms:
ABH8, TRM9, MRT71, TRMT9, TRMT9A, ALKBH8
Research Use:
Cell Biology
Frequently bought together:

Description

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ALKBH8 Antibody (CAB7142)

The ALKBH8 Antibody (CAB7142) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, has high specificity and sensitivity for detecting ALKBH8 in human samples, making it ideal for use in Western blot applications.ALKBH8, a member of the AlkB family of proteins, plays a crucial role in cellular processes such as DNA repair, RNA metabolism, and gene expression regulation.

This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:ALKBH8 Antibody
SKU:CAB7142
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MDSN HQSN YKLS KTEK KFLR KQIK AKHT LLRH EGIE TVSY ATQS LVVA NGGL GNGV SRNQ LLPV LEKC GLVD ALLM PPNK PYSF ARYR TTEE SKRA YVTL NGKE VVDD LGQK ITLY LNFV EKVQ WKEL RPQA LPPG LMVV EEII SSEE EKML LESV DWTE DTDN QNSQ KSLK HRRV KHFG YEFH YENN NVDK DKPL SGGL PDIC ESFL EKWL RKGY IKHK PDQM TINQ YE
Tested Applications:WB IHC-P ELISA
Recommended Dilution:
WB1:2000 - 1:10000
IHC-P1:100 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:ABH8, TRM9, MRT71, TRMT9, TRMT9A, ALKBH8
Positive Sample:A549, Rat brain
Cellular Localization:Cytoplasm, Nucleus.
Calculated MW:75kDa
Observed MW:75kDa

Enables tRNA (uracil) methyltransferase activity; tRNA binding activity; and zinc ion binding activity. Involved in cellular response to DNA damage stimulus; tRNA methylation; and tRNA wobble uridine modification. Located in cytosol and nuclear body. Implicated in autosomal recessive non-syndromic intellectual disability.

Purification MethodAffinity purification
Gene ID91801
RRIDAB_2767697
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates, using ALKBH8 Rabbit pAb (CAB7142) at 1:14000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Mouse lung using ALKBH8 Rabbit pAb (CAB7142) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
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