Cell Biology Antibodies 11
Anti-ALKBH8 Antibody (CAB7142)
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- SKU:
- CAB7142
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-ALKBH8 Antibody |
| Antibody SKU: | CAB7142 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human ALKBH8 (NP_620130.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:100 - 1:200 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse |
| Positive Samples: | A-549, NCI-H460, U-87MG, HeLa, K-562 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human ALKBH8 (NP_620130.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MDSN HQSN YKLS KTEK KFLR KQIK AKHT LLRH EGIE TVSY ATQS LVVA NGGL GNGV SRNQ LLPV LEKC GLVD ALLM PPNK PYSF ARYR TTEE SKRA YVTL NGKE VVDD LGQK ITLY LNFV EKVQ WKEL RPQA LPPG LMVV EEII SSEE EKML LESV DWTE DTDN QNSQ KSLK HRRV KHFG YEFH YENN NVDK DKPL SGGL PDIC ESFL EKWL RKGY IKHK PDQM TINQ YE |
| Gene ID: | 91801 |
| Uniprot: | Q96BT7 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 25kDa/27kDa/75kDa |
| Observed MW: | 75kDa |
| Synonyms: | ALKBH8, ABH8, TRM9, TRMT9 |
| Background: |
| UniProt Protein Function: | ALKBH8: Catalyzes the methylation of 5-carboxymethyl uridine to 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in tRNA. Catalyzes the last step in the formation of 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in target tRNA. Has a preference for tRNA(Arg) and tRNA(Glu), and does not bind tRNA(Lys). Required for normal survival after DNA damage. May inhibit apoptosis and promote cell survival and angiogenesis. Belongs to the alkB family. 4 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:RNA processing; Methyltransferase; EC 2.1.1.229 Chromosomal Location of Human Ortholog: 11q22.3 Cellular Component: microtubule cytoskeleton; nucleoplasm; nucleus; cytosol Molecular Function:protein binding; zinc ion binding; tRNA (uracil) methyltransferase activity; iron ion binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors; tRNA binding Biological Process: tRNA wobble uridine modification; response to DNA damage stimulus; tRNA methylation |
| UniProt Code: | Q96BT7 |
| NCBI GenInfo Identifier: | 189027650 |
| NCBI Gene ID: | 91801 |
| NCBI Accession: | Q96BT7.2 |
| UniProt Secondary Accession: | Q96BT7,Q8N989, B1Q2M0, B4DEF6, |
| UniProt Related Accession: | Q96BT7 |
| Molecular Weight: | 664 |
| NCBI Full Name: | Alkylated DNA repair protein alkB homolog 8 |
| NCBI Synonym Full Names: | alkB, alkylation repair homolog 8 (E. coli) |
| NCBI Official Symbol: | ALKBH8 |
| NCBI Official Synonym Symbols: | ABH8 |
| NCBI Protein Information: | alkylated DNA repair protein alkB homolog 8; AlkB homologue 8; probable alpha-ketoglutarate-dependent dioxygenase ABH8; tRNA (carboxymethyluridine(34)-5-O)-methyltransferase ABH8; S-adenosyl-L-methionine-dependent tRNA methyltransferase ABH8 |
| UniProt Protein Name: | Alkylated DNA repair protein alkB homolog 8 |
| UniProt Synonym Protein Names: | Probable alpha-ketoglutarate-dependent dioxygenase ABH8; S-adenosyl-L-methionine-dependent tRNA methyltransferase ABH8; tRNA (carboxymethyluridine(34)-5-O)-methyltransferase ABH8 (EC:2.1.1.229) |
| Protein Family: | Alkylated DNA repair protein |
| UniProt Gene Name: | ALKBH8 |
| UniProt Entry Name: | ALKB8_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ALKBH8 antibody (CAB7142) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded mouse lung using ALKBH8 antibody (CAB7142) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human gastric cancer using ALKBH8 antibody (CAB7142) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of A549 cells using ALKBH8 antibody (CAB7142). Blue: DAPI for nuclear staining. |
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