Description
ALK Antibody (CAB0766)
The ALK Antibody (CAB0766) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits strong reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the ALK protein, this antibody enables accurate detection and analysis in a variety of cell types, making it an excellent tool for studies in cancer biology and drug development.ALK, also known as anaplastic lymphoma kinase, is a key player in signaling pathways that regulate cell proliferation and survival. Dysregulation of ALK has been implicated in various cancers, including anaplastic large cell lymphoma and non-small cell lung carcinoma.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
| Product Name: | ALK Antibody |
| SKU: | CAB0766 |
| Size: | 20μL, 100μL |
| Reactivity: | Human, Mouse |
| Conjugate: | Unconjugated |
| Immunogen: | Synthetic peptide. This information is considered to be commercially sensitive. | ||||||
| Sequence: | PIAK KEPH DRGN LGLE GSCT VPPN VATG RLPG ASLL LEPS SLTA NMKE VPLF RLRH FPCG NVNY GYQQ QGLP LEAA TAPG AGHY EDTI LKSK NSMN QPGP | ||||||
| Tested Applications: | WB IF/ICC ELISA | ||||||
| Recommended Dilution: |
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| Synonyms: | ALK1, CD246, NBLST3, ALK |
| Positive Sample: | PC-3, Mouse testis |
| Cellular Localization: | Cell Membrane, Single-Pass Type I Membrane Protein. |
| Calculated MW: | 176kDa |
| Observed MW: | 140kDa/220kDa |
This gene encodes a receptor tyrosine kinase, which belongs to the insulin receptor superfamily. This protein comprises an extracellular domain, an hydrophobic stretch corresponding to a single pass transmembrane region, and an intracellular kinase domain. It plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system. This gene has been found to be rearranged, mutated, or amplified in a series of tumours including anaplastic large cell lymphomas, neuroblastoma, and non-small cell lung cancer. The chromosomal rearrangements are the most common genetic alterations in this gene, which result in creation of multiple fusion genes in tumourigenesis, including ALK (chromosome 2)/EML4 (chromosome 2), ALK/RANBP2 (chromosome 2), ALK/ATIC (chromosome 2), ALK/TFG (chromosome 3), ALK/NPM1 (chromosome 5), ALK/SQSTM1 (chromosome 5), ALK/KIF5B (chromosome 10), ALK/CLTC (chromosome 17), ALK/TPM4 (chromosome 19), and ALK/MSN (chromosome X).
| Purification Method | Affinity purification |
| Gene ID | 238 |
| RRID | AB_2757389 |
| Buffer Information | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3. |
 | Western blot analysis of lysates from SH-SY5Y cells, using ALK Rabbit pAb (CAB0766) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s. |
 | Immunofluorescence analysis of SH-SY5Y cells using ALK Rabbit pAb (CAB0766) at dilution of 1:200. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
