Cell Death Antibodies 1

AIFM2 Rabbit Polyclonal Antibody (CAB12128)

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SKU:
CAB12128
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Death
Reactivity:
Human, Mouse, Rat
Synonyms:
AMID, FSP1, PRG3, AIFM2/FSP1
Research Use:
Cell Death
Frequently bought together:

Description

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AIFM2 Antibody (CAB12128)

The AIFM2 Antibody (CAB12128) is a high-quality antibody developed for reliable detection and analysis of target proteins. This rabbit-derived antibody is highly specific to human samples and has been validated for use in Western blot applications. By targeting the AIFM2 protein, this antibody allows for precise detection and analysis in various cell types, making it ideal for studies related to mitochondrial dysfunction, metabolism, and cell death pathways.AIFM2, also known as a putative mitochondrial inner membrane protein, plays a critical role in maintaining mitochondrial integrity and function.

This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:AIFM2 Antibody
SKU:CAB12128
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:KDSF HHNV AALR ASVE TGFA KKTF ISYS VTFK DNFR QGLV VGID LKNQ MVLL QGGE ALPF SHLI LATG STGP FPGK FNEV SSQQ AAIQ AYED MVRQ VQRS RFIV VVGG GSAG VEMA AEIK TEYP EKEV TLIH SQVA LADK ELLP SVRQ EVKE ILLR KGVQ LLLS ERVS NLEE LPLN EYRE YIKV QTDK GTEV ATNL VILC TGIK INSS AYRK AFES RLAS SGAL RVNE HLQV EGHS NVYA IGDC ADVR TPKM AYLA GLHA NIAV ANIV NSVK QRPL QAYK PGAL TFLL SMGR NDGV GQIS GFYV GRLM VRLT KSRD LFVS TSWK TMRQ S
Tested Applications:WB IHC-P ELISA
Recommended Dilution:
WB1:500 - 1:1000
IHC-P1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:AMID, FSP1, PRG3, AIFM2/FSP1
Positive Sample:HepG2, Mouse liver, Mouse testis, Rat liver, Rat testis
Cellular Localization:Cytoplasm, Lipid-Anchor, Membrane, Mitochondrion Outer Membrane.
Calculated MW:41kDa
Observed MW:41kDa

This gene encodes a flavoprotein oxidoreductase that binds single stranded DNA and is thought to contribute to apoptosis in the presence of bacterial and viral DNA. The expression of this gene is also found to be induced by tumor suppressor protein p53 in colon cancer cells.

Purification MethodAffinity purification
Gene ID84883
RRIDAB_2759017
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of lysates from HepG2 cells, using AIFM2/FSP1 Rabbit pAb (CAB12128) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 180s.
Product ImageWestern blot analysis of various lysates using AIFM2/FSP1 Rabbit pAb (CAB12128) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 60s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human liver cancer using AIFM2/FSP1 Rabbit pAb (CAB12128) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
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