Cell Biology Antibodies 6

Angiotensinogen Rabbit Polyclonal Antibody (CAB15637)

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SKU:
CAB15637
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Reactivity:
Human, Mouse, Rat
Synonyms:
ANHU, hFLT1, SERPINA8, Angiotensinogen
Research Use:
Cell Biology
Frequently bought together:

Description

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Angiotensinogen Antibody (CAB15637)

The Angiotensinogen Antibody (CAB15637) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity towards human samples and is specifically validated for Western blot applications. By binding to the CD300A protein, researchers can accurately detect and analyze its expression in various cell types, making it particularly useful for studies in immunology and cancer research.CD300A, also known as an immune inhibitory receptor, plays a key role in immune homeostasis by regulating inflammation and suppressing allergic reactions.

This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:Angiotensinogen Antibody
SKU:CAB15637
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:RVYI HPFH LVIH NEST CEQL AKAN AGKP KDPT FIPA PIQA KTSP VDEK ALQD QLVL VAAK LDTE DKLR AAMV GMLA NFLG FRIY GMHS ELWG VVHG ATVL SPTA VFGT LASL YLGA LDHT ADRL QAIL GVPW KDKN CTSR LDAH KVLS ALQA VQGL LVAQ GRAD SQAQ LLLS TVVG VFTA PGLH LKQP FVQG LALY TPVV LPRS LDFT ELDV AAEK IDRF MQAV TGWK TGCS LMGA SVDS TLAF NTYV HFQG KMKG FSLL AEPQ EFWV DNST SVSV PMLS GMGT FQHW SDIQ DNFS VTQV PFTE SACL LLIQ PHYA SDLD KVEG LTFQ QNSL NWMK KLSP RTIH LTMP QLVL QGSY DLQD LLAQ AELP AILH TELN LQKL SNDR IRVG EVLN SIFF ELEA DERE PTES TQQL NKPE VLEV TLNR PFLF AVYD QSAT ALHF LGRV ANPL STA
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:1000
IHC-P1:50 - 1:100
IF/ICC1:50 - 1:100
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:ANHU, hFLT1, SERPINA8, Angiotensinogen
Positive Sample:Human plasma, Mouse plasma
Cellular Localization:Secreted.
Calculated MW:53kDa
Observed MW:53kDa

The protein encoded by this gene, pre-angiotensinogen or angiotensinogen precursor, is expressed in the liver and is cleaved by the enzyme renin in response to lowered blood pressure. The resulting product, angiotensin I, is then cleaved by angiotensin converting enzyme (ACE) to generate the physiologically active enzyme angiotensin II. The protein is involved in maintaining blood pressure, body fluid and electrolyte homeostasis, and in the pathogenesis of essential hypertension and preeclampsia. Mutations in this gene are associated with susceptibility to essential hypertension, and can cause renal tubular dysgenesis, a severe disorder of renal tubular development. Defects in this gene have also been associated with non-familial structural atrial fibrillation, and inflammatory bowel disease.

Purification MethodAffinity purification
Gene ID183
RRIDAB_2763043
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using Angiotensinogen Rabbit pAb (CAB15637) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human liver cancer using Angiotensinogen Rabbit pAb (CAB15637) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunofluorescence analysis of HepG2 cells using Angiotensinogen Rabbit pAb (CAB15637) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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