Cell Biology Antibodies 4

ADH5 Rabbit Polyclonal Antibody (CAB13459)

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SKU:
CAB13459
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Reactivity:
Human, Mouse, Rat
Synonyms:
FDH, ADHX, ADH-3, AMEDS, BMFS7, FALDH, GSNOR, GSH-FDH, HEL-S-60p, ADH5/GSNOR
Research Use:
Cell Biology
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Description

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ADH5 Antibody (CAB13459)

The ADH5 Antibody (CAB13459) is a high-quality antibody developed for reliable detection and analysis of target proteins. The antibody is produced in rabbits and has been shown to have high reactivity with human samples, making it ideal for use in Western blot applications. By binding to the ADH5 protein, this antibody allows for the detection and analysis of ADH5 in various cell types, making it a versatile option for researchers in immunology and cancer research.ADH5, also known as alcohol dehydrogenase 5, is an enzyme that plays a crucial role in the metabolism of alcohol and other toxic substances in the liver.

This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:ADH5 Antibody
SKU:CAB13459
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MANE VIKC KAAV AWEA GKPL SIEE IEVA PPKA HEVR IKII ATAV CHTD AYTL SGAD PEGC FPVI LGHE GAGI VESV GEGV TKLK AGDT VIPL YIPQ CGEC KFCL NPKT NLCQ KIRV TQGK GLMP DGTS RFTC KGKT ILHY MGTS TFSE YTVV ADIS VAKI DPLA PLDK VCLL GCGI STGY GAAV NTAK LEPG SVCA VFGL GGVG LAVI MGCK VAGA SRII GVDI NKDK FARA KEFG ATEC INPQ DFSK PIQE VLIE MTDG GVDY SFEC IGNV KVMR AALE ACHK GWGV SVVV GVAA SGEE IATR PFQL VTGR TWKG TAFG GWKS VESV PKLV SEYM SKKI KVDE FVTH NLSF DEIN KAFE LMHS GKSI RTVV KI
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:1000
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:FDH, ADHX, ADH-3, AMEDS, BMFS7, FALDH, GSNOR, GSH-FDH, HEL-S-60p, ADH5/GSNOR
Positive Sample:K-562, Mouse brain, Rat liver, Rat kidney
Cellular Localization:Cytoplasm.
Calculated MW:40kDa
Observed MW:40kDa

This gene encodes a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. The encoded protein forms a homodimer. It has virtually no activity for ethanol oxidation, but exhibits high activity for oxidation of long-chain primary alcohols and for oxidation of S-hydroxymethyl-glutathione, a spontaneous adduct between formaldehyde and glutathione. This enzyme is an important component of cellular metabolism for the elimination of formaldehyde, a potent irritant and sensitizing agent that causes lacrymation, rhinitis, pharyngitis, and contact dermatitis. The human genome contains several non-transcribed pseudogenes related to this gene.

Purification MethodAffinity purification
Gene ID128
RRIDAB_2760319
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using ADH5/GSNOR Rabbit pAb (CAB13459) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Product ImageImmunofluorescence analysis of HeLa cells using ADH5/GSNOR Rabbit pAb (CAB13459) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Product ImageImmunofluorescence analysis of NIH/3T3 cells using ADH5/GSNOR Rabbit pAb (CAB13459) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Product ImageImmunofluorescence analysis of PC-12 cells using ADH5/GSNOR Rabbit pAb (CAB13459) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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