Epigenetics & Nuclear Signaling Antibodies 2

Anti-Acetyl-Histone H3-K4 Antibody (CAB16078)

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SKU:
CAB16078
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-Acetyl-Histone H3-K4 Antibody
Antibody SKU:CAB16078
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat, Other (Wide Range)
Host Species:Rabbit
Immunogen:A synthetic acetylated peptide around K4 of human Histone H3 (NP_003484.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:Human, Mouse, Rat, Other (Wide Range)
Positive Samples:NIH/3T3, C6
Immunogen:A synthetic acetylated peptide around K4 of human Histone H3 (NP_003484.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:ARTK Q
Gene ID:8290
Uniprot:Q16695
Cellular Location:Chromosome, Nucleus
Calculated MW:15kDa
Observed MW:15kDa/17kDa
Synonyms:H3.4, H3/g, H3FT, H3t, HIST3H3, Histone H3, HIST1H3A
Background:Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
UniProt Protein Function:HIST3H3: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Expressed in testicular cells. Belongs to the histone H3 family.
UniProt Protein Details:

Protein type:DNA-binding

Chromosomal Location of Human Ortholog: 1q42

Cellular Component: nucleoplasm; nucleus

Molecular Function:protein binding; DNA binding; histone binding; protein heterodimerization activity

Biological Process: nucleosome assembly; negative regulation of protein oligomerization; protein heterotetramerization; telomere maintenance

NCBI Summary:Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Jul 2008]
UniProt Code:Q16695
NCBI GenInfo Identifier:18202512
NCBI Gene ID:8290
NCBI Accession:Q16695.3
UniProt Secondary Accession:Q16695,Q6FGU4, B2R5K3,
UniProt Related Accession:Q16695
Molecular Weight:
NCBI Full Name:Histone H3.1t
NCBI Synonym Full Names:histone cluster 3, H3
NCBI Official Symbol:HIST3H3  
NCBI Official Synonym Symbols:H3t; H3.4; H3/g; H3FT  
NCBI Protein Information:histone H3.1t; H3/t; histone 3, H3; H3 histone family, member T
UniProt Protein Name:Histone H3.1t
UniProt Synonym Protein Names:H3/g
UniProt Gene Name:HIST3H3  
UniProt Entry Name:H31T_HUMAN
Western blot - Acetyl-Histone H3-K4 Rabbit pAb Western blot analysis of extracts of various cell lines, using Acetyl-Histone H3-K4 antibody at 1:1000 dilution. NIH/3T3 cells were treated by TSA (1 uM) at 37°C for 18 hours. C6 cells were treated by TSA (1 uM) at 37°C for 18 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Western blot - Acetyl-Histone H3-K4 Rabbit pAb Western blot analysis of extracts of NIH/3T3 cells, using Acetyl-Histone H3-K4 antibody at 1:1000 dilution. NIH/3T3 cells were treated by TSA (1 uM) at 37°C for 18 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 300s.
Immunofluorescence - Acetyl-Histone H3-K4 Rabbit pAb Immunofluorescence analysis of C6 cells using Acetyl-Histone H3-K4 antibody at dilution of 1:100. C6 cells were treated by TSA (1 uM) at 37°C for 18 hours. Blue: DAPI for nuclear staining.
Immunofluorescence - Acetyl-Histone H3-K4 Rabbit pAb Immunofluorescence analysis of HeLa cells using Acetyl-Histone H3-K4 antibody at dilution of 1:100. HeLa cells were treated by TSA (1 uM) at 37°C for 18 hours. Blue: DAPI for nuclear staining.
Immunofluorescence - Acetyl-Histone H3-K4 Rabbit pAb Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H3-K4 antibody at dilution of 1:100. NIH/3T3 cells were treated by TSA (1 uM) at 37°C for 18 hours. Blue: DAPI for nuclear staining.
Chromatin Immunoprecipitation - Acetyl-Histone H3-K4 Rabbit pAb Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Acetyl-Histone H3-K4 antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
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