Epigenetics & Nuclear Signaling Antibodies 4

Acetyl-Histone H3-K27 Rabbit Polyclonal Antibody (CAB7253)

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SKU:
CAB7253
Product Type:
Antibody
Applications:
WB
Applications:
IHC
Applications:
IF
Applications:
IP
Applications:
ChIP
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
Reactivity:
Human, Mouse, Rat, Other (Wide Range Predicted)
Synonyms:
H3t, H3.4, H3/g, H3FT, H3C16, HIST3H3, Acetyl-Histone H3-K27
Research Use:
Epigenetics and Nuclear Signaling
Frequently bought together:

Description

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Acetyl-Histone H3-K27 Antibody (CAB7253)

The Acetyl-Histone H3-K27 Antibody (CAB7253) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to acetylated Histone H3 at lysine 27, this antibody enables researchers to detect and analyze this modification in various cell types, making it ideal for epigenetics and cancer research studies.

This antibody is validated for use in WB, IHC-P, IF/ICC, IP, ChIP, ChIP-seq, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat, Other (Wide Range Predicted) samples.

Product Name:Acetyl-Histone H3-K27 Antibody
SKU:CAB7253
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Conjugate:Unconjugated
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:MART KQTA RKST GGKA PRKQ LATK AARK SAPA TGGV KKPH RYRP GTVA LREI RRYQ KSTE LLIR KLPF QRLV REIA QDFK TDLR FQSS AVMA LQEA CEAY
Tested Applications:WB IHC-P IF/ICC IP ChIP ChIP-seq ELISA
Recommended Dilution:
WB1:1000 - 1:5000
IHC-P1:50 - 1:200
IF/ICC1:50 - 1:200
IP0.5ug-4ug antibody for 200ug-400ug extracts of whole cells
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
ChIP5μg antibody for 5μg-10μg of Chromatin
ChIP-seq1:20 - 1:100
Synonyms:H3t, H3.4, H3/g, H3FT, H3C16, HIST3H3, Acetyl-Histone H3-K27
Positive Sample:NIH/3T3 treated with TSA, C6 treated with TSA
Cellular Localization:Chromosome, Nucleus.
Calculated MW:15kDa
Observed MW:17kDa

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.

Purification MethodAffinity purification
Gene ID8290 8350
RRIDAB_2767797
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of lysates from NIH/3T3 cells, using Acetyl-Histone H3-K27 Rabbit pAb (CAB7253) at 1:2000 dilution. NIH/3T3 cells were treated with TSA (1 uM) at 37℃ for 18 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Product ImageWestern blot analysis of lysates from C6 cells, using Acetyl-Histone H3-K27 Rabbit pAb (CAB7253) at 1:2000 dilution. C6 cells were treated with TSA (1 uM) at 37℃ for 18 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human spleen using Acetyl-Histone H3-K27 Rabbit pAb (CAB7253) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Mouse lung using Acetyl-Histone H3-K27 Rabbit pAb (CAB7253) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunofluorescence analysis of C6 cells treated with TSA (upper left) and untreated C6 cells (upper right) using Acetyl-Histone H3-K27 Rabbit pAb (red, CAB7253) at dilution of 1:100. Blue: DAPI for nuclear staining.
Product ImageImmunofluorescence analysis of NIH-3T3 cells treated with TSA (upper left) and untreated NIH-3T3 cells(upper right) using Acetyl-Histone H3-K27 Rabbit pAb (red, CAB7253) at dilution of 1:100. Blue: DAPI for nuclear staining.
Product ImageChromatin immunoprecipitation was performed with cross-linked chromatin from 293T, using Acetyl-Histone H3-K27 Rabbit pAb antibody (CAB7253) and rabbit IgG(AC005).The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram constructed the ratios of the ratio of the immunoprecipitated DNA versus the input.
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