The Alpha-tubulin (ubiquitous) chain Rabbit pAb (CABC025) is a high-quality antibody developed for reliable detection and analysis of target proteins. This rabbit polyclonal antibody is highly specific and sensitive, making it ideal for use in Western blot applications for the detection and analysis of alpha tubulin in human samples.Alpha tubulin is a key component of microtubules, essential structures for cell division, intracellular transport, and cell motility. Dysregulation of alpha tubulin has been implicated in various diseases, including cancer and neurodegenerative disorders.
This antibody is validated for use in WB, IF/ICC applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Alpha-tubulin (ubiquitous) chain Rabbit pAb
SKU:
CABC025
Size:
100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
HeLa, PC-12, A-549, NIH/3T3, U-251MG, Mouse brain , Rat brain
Cellular Localization:
Cytoplasm, Cytoskeleton.
Calculated MW:
50kDa
Observed MW:
50kDa
Enables double-stranded RNA binding activity and ubiquitin protein ligase binding activity. Predicted to be involved in microtubule cytoskeleton organization and mitotic cell cycle. Predicted to act upstream of or within cellular response to interleukin-4. Located in microtubule.
Purification Method
Affinity purification
Gene ID
10376
RRID
AB_2768344
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Alpha-tubulin (ubiquitous) chain Rabbit pAb (CABC025) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunofluorescence analysis of U2OS cells using Alpha-tubulin (ubiquitous) chain Rabbit pAb (CABC025) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of C6 cells using Alpha-tubulin (ubiquitous) chain Rabbit pAb (CABC025) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
