β-Tubulin Rabbit Polyclonal Antibody (CABC008)

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SKU:
CABC008
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Human, Mouse, Rat
Host Species:
Rabbit
Synonyms:
M40, TUBB1, TUBB5, CDCBM6, CSCSC1, OK/SW-cl.56, β-Tubulin
Frequently bought together:

Description

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β-Tubulin Rabbit pAb (CABC008)

The β-Tubulin Rabbit pAb (CABC008) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly reactive with tubulin in various species and is validated for use in Western blot and immunofluorescence applications.Tubulin is essential for cell division, forming the structural component of microtubules that are critical for various cellular processes such as cell division, intracellular transport, and cell shape maintenance.

This antibody is validated for use in WB, IHC-P, IF/ICC applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:β-Tubulin Rabbit pAb
SKU:CABC008
Size:100μL, 200μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:SDLQ LERI SVYY NEAS SHKY VPRA ILVD LEPG TMDS VRSG AFGH LFRP DNFI FGQS GAGN NWAK GHYT EGAE LVDS VLDV VRKE CENC DCLQ GFQL THSL GGGT GSGM GTLL ISKV REEY PDRI MNTF SVVP SPKV SDTV VEPY NATL SIHQ LVEN TDET YCID NEAL YDIC FRTL KLAT PTYG DLNH LVSA TMSG VTTS LRFP GQLN ADLR KLAV NMVP F
Tested Applications:WB IHC-P IF/ICC
Recommended Dilution:
WB1:2000 - 1:10000
IHC-P1:50 - 1:200
IF/ICC1:50 - 1:200
Synonyms:M40, TUBB1, TUBB5, CDCBM6, CSCSC1, OK/SW-cl.56, β-Tubulin
Positive Sample:293T, SH-SY5Y, Mouse brain, Rat brain
Cellular Localization:Cytoplasm, Cytoskeleton.
Calculated MW:50kDa
Observed MW:55kDa

This gene encodes a beta tubulin protein. This protein forms a dimer with alpha tubulin and acts as a structural component of microtubules. Mutations in this gene cause cortical dysplasia, complex, with other brain malformations 6. Alternative splicing results in multiple splice variants. There are multiple pseudogenes for this gene on chromosomes 1, 6, 7, 8, 9, and 13.

Purification MethodAffinity purification
Gene ID203068
RRIDAB_2773006
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates, using β-Tubulin Rabbit pAb (CABC008) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human liver using β-Tubulin Rabbit pAb (CABC008) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunofluorescence analysis of C6 cells using β-Tubulin Rabbit pAb (CABC008) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Product ImageImmunofluorescence analysis of NIH-3T3 cells using β-Tubulin Rabbit pAb (CABC008) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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