AFG3L2 Polyclonal Antibody (CAB23598)

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SKU:
CAB23598
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Human, Mouse, Rat
Host Species:
Rabbit
Synonyms:
OPA12, SCA28, SPAX5, AFG3L2
Frequently bought together:

Description

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AFG3L2 Polyclonal Antibody (CAB23598)

The AFG3L2 Polyclonal Antibody (CAB23598) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific and reactive with human samples, making it suitable for use in Western blot applications. By targeting the AFG3L2 protein, this antibody enables precise detection and analysis in various cell types, aiding in investigations related to mitochondrial biology, neurodegenerative diseases, and protein degradation pathways.AFG3L2 is a conserved mitochondrial ATP-dependent metalloprotease, essential for maintaining mitochondrial homeostasis and cellular function.

This antibody is validated for use in WB, IHC-P, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:AFG3L2 Polyclonal Antibody
SKU:CAB23598
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:DSIN QKHF EQAI ERVI GGLE KKTQ VLQP EEKK TVAY HEAG HAVA GWYL EHAD PLLK VSII PRGK GLGY AQYL PKEQ YLYT KEQL LDRM CMTL GGRV SEEI FFGR ITTG AQDD LRKV TQSA YAQI VQFG MNEK VGQI SFDL PRQG DMVL EKPY SEAT ARLI DDEV RILI NDAY KRTV ALLT EKKA DVEK VALL LLEK EVLD KNDM VELL GPRP FAEK STYE EFVE GTGS LDED TSLP EGLK DWNK EREK EKEE PPGE KVAN
Tested Applications:WB IHC-P IP ELISA
Recommended Dilution:
WB1:500 - 1:1000
IHC-P1:50 - 1:200
IP0.5μg-4μg antibody for 400μg-600μg extracts of whole cells
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:OPA12, SCA28, SPAX5, AFG3L2
Positive Sample:Jurkat, 293T, Mouse liver
Cellular Localization:Mitochondrion Membrane, Multi-Pass Membrane Protein.
Calculated MW:89kDa
Observed MW:80kDa

This gene encodes a protein localized in mitochondria and closely related to paraplegin. The paraplegin gene is responsible for an autosomal recessive form of hereditary spastic paraplegia. This gene is a candidate gene for other hereditary spastic paraplegias or neurodegenerative disorders.

Purification MethodAffinity purification
Gene ID10939
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates, using AFG3L2 Rabbit pAb (CAB23598) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using AFG3L2 Rabbit pAb (CAB23598) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
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