VISTA / B7-H5 / GI24 Polyclonal Antibody (CAB24535)
The VISTA / B7-H5 / GI24 Polyclonal Antibody (CAB24535) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, developed through the immunization of rabbits, has been shown to be highly reactive with human samples and is specifically validated for use in Western blot applications.The VISTA/B7-H5/GI24 protein, also known as V-domain Ig suppressor of T cell activation (VISTA), plays a critical role in immune homeostasis by inhibiting T cell activation and promoting immune tolerance. Its significance in immune regulation makes it a promising target for research into diseases such as cancer, autoimmune disorders, and chronic inflammatory conditions.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Rat samples.
Product Name:
VISTA / B7-H5 / GI24 Polyclonal Antibody
SKU:
CAB24535
Size:
20μL, 100μL
Reactivity:
Human, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Cell Membrane, Single-Pass Type I Membrane Protein.
Calculated MW:
33kDa
Observed MW:
45-65kDa
Enables endopeptidase activator activity; enzyme binding activity; and identical protein binding activity. Involved in several processes, including negative regulation of cytokine production; positive regulation of macromolecule metabolic process; and regulation of T cell activation. Located in plasma membrane.
Purification Method
Affinity purification
Gene ID
64115
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from HLE cells, using VISTA / B7-H5 / GI24 Rabbit pAb (CAB24535) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Western blot analysis of lysates from Rat spleen, using VISTA / B7-H5 / GI24 Rabbit pAb (CAB24535) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Immunohistochemistry analysis of paraffin-embedded Rat spleen tissue using VISTA / B7-H5 / GI24 Rabbit pAb (CAB24535) at a dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
