Enzyme-linked immunosorbent assay (ELISA) for the quantitative determination of Ustekinumab (Stelara®) in human serum and plasma.This Assay Genie kit has been especially developed for the quantitative analysis of Ustekinumab in serum and plasma samples.

Solid phase enzyme-linked immunosorbent assay (ELISA) based on the sandwich principle. Standards and samples (serum or plasma) are incubated in the microtitre plate coated with the reactant for Ustekinumab (Stelara®). After incubation, the wells are washed. A horse radish peroxidase (HRP) conjugated probe is added and binds to ustekinumab captured by the reactant on the surface of the wells. Following incubation wells are washed and the bound enzymatic activity is detected by addition of chromogen-substrate. The colour developed is proportional to the amount of ustekinumab in the sample or standard. Results of samples can be determined directly using the standard curve.

Ustekinumab is a fully human monoclonal antibody that binds with high specificity and affinity to the cytokines interleukin (IL)-12 and IL-23, thereby suppressing IL-12- and IL-23-mediated inflammation associated with psoriasis. Ustekinumab is a human IgG1-kappa monoclonal antibody designed to interfere with the triggering of the body's inflammatory response through the suppression of certain cytokines. Specifically, ustekinumab blocks interleukin IL-12 and IL-23 by binding with high affinity and specificity to the p40 subunit of IL-12 and IL-23, therefore disrupting the proinflammatory pathway that contributes to several chronic diseases. In a more minor role, ustekinumab also interferes with the expression of monocyte chemotactic protein-1 (MCP-1), tumor necrosis factor-alpha (TNF-alpha), interferon-inducible protein-10 (IP-10), and interleukin IL-8.

Ustekinumab is used as a treatment for plaque psoriasis and a particular type of arthritis (psoriatic arthritis). Furthermore, ustekinumab is used for certain bowel conditions including Crohn's disease and ulcerative colitis.

Ustekinumab (STELARA®) is indicated for the treatment of adult patients with moderate to severe plaque psoriasis (Ps) who are candidates for phototherapy or systemic therapy. It works by blocking certain natural proteins in your body (interleukin-12 and interleukin-23) that cause inflammation (swelling) in these conditions. Ustekinumab (STELARA®) is the only FDA-approved medicine that targets IL-12 and IL-23, which are thought to be associated with gastrointestinal inflammation in Crohn's disease.

Stelara® is a registered trademark of Johnson and Johnson, Inc.