Antibody Name:	USP2 Antibody (PACO15604)
Antibody SKU:	PACO15604
Size:	50ul
Host Species:	Rabbit
Tested Applications:	ELISA, IHC
Recommended Dilutions:	ELISA:1:1000-1:2000, IHC:1:25-1:100
Species Reactivity:	Human, Mouse, Rat
Immunogen:	Fusion protein of human USP2

Form:	Liquid
Storage Buffer:	-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:	Antigen affinity purification
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

	The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using PACO15604(USP2 Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: x200).
	The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using PACO15604(USP2 Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: x200).

Background:

This gene encodes a member of the family of de-ubiquitinating enzymes, which belongs to the peptidase C19 superfamily. The encoded protein is a ubiquitin-specific protease which is required for TNF-α (tumor necrosis factor alpha) -induced NF-kB (nuclear factor kB) signaling. This protein deubiquitinates polyubiquitinated target proteins such as fatty acid, synthase, murine double minute 2 (MDM2), MDM4/MDMX and cyclin D1. MDM2 and MDM4 are negative regulators of the p53 tumor suppressor and cyclin D1 is required for cell cycle G1/S transition. Multiple alternatively spliced transcript variants encoding different isoforms have been identified.

Synonyms:

ubiquitin specific peptidase 2

UniProt Protein Function:	USP2: Hydrolase that deubiquitinates polyubiquitinated target proteins such as MDM2, MDM4 and CCND1. Isoform 1 and isoform 4 possess both ubiquitin-specific peptidase and isopeptidase activities. Deubiquitinates MDM2 without reversing MDM2-mediated p53/TP53 ubiquitination and thus indirectly promotes p53/TP53 degradation and limits p53 activity. Has no deubiquitinase activity against p53/TP53. Prevents MDM2-mediated degradation of MDM4. Plays a role in the G1/S cell-cycle progression in normal and cancer cells. Plays a role in the regulation of myogenic differentiation of embryonic muscle cells. Homooligomer. Found in trimeric complex with MDM2 and MDM4 and UPB2. Interacts with CCND1; the interaction is direct and promotes its stabilization by antagonizing ubiquitin-dependent degradation. Interacts (via N-terminus and C- terminus) with MDM2. Interacts with MDM4. Down-regulated by cisplatin. Expressed in mesangial cells of the kidney and in different types of glomerulonephritides. Cleavage is inhibited by ubiquitin in a dosage- dependent manner. Cleavage is blocked by ubiquitin aldehyde. Belongs to the peptidase C19 family. USP2 subfamily. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Ubiquitin-specific protease; Protease; EC 3.4.19.12 Chromosomal Location of Human Ortholog: 11q23.3 Cellular Component: centrosome; perinuclear region of cytoplasm; cytoplasm; cell cortex; nucleus Molecular Function:identical protein binding; protein binding; cyclin binding; ubiquitin protein ligase binding; metal ion binding; cysteine-type endopeptidase activity; ubiquitin-specific protease activity Biological Process: proteasomal ubiquitin-dependent protein catabolic process; negative regulation of skeletal muscle development; muscle development; protein deubiquitination; protein stabilization; positive regulation of skeletal muscle development; negative regulation of transcription from RNA polymerase II promoter; positive regulation of mitotic cell cycle; cell cycle; circadian behavior; entrainment of circadian clock by photoperiod; locomotor rhythm; circadian regulation of gene expression
NCBI Summary:	This gene encodes a member of the family of de-ubiquitinating enzymes, which belongs to the peptidase C19 superfamily. The encoded protein is a ubiquitin-specific protease which is required for TNF-alpha (tumor necrosis factor alpha) -induced NF-kB (nuclear factor kB) signaling. This protein deubiquitinates polyubiquitinated target proteins such as fatty acid synthase, murine double minute 2 (MDM2), MDM4/MDMX and cyclin D1. MDM2 and MDM4 are negative regulators of the p53 tumor suppressor and cyclin D1 is required for cell cycle G1/S transition. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011]
UniProt Code:	O75604
NCBI GenInfo Identifier:	20141855
NCBI Gene ID:	9099
NCBI Accession:	O75604.2
UniProt Secondary Accession:	O75604,Q8IUM2, Q8IW04, Q96MB9, Q9BQ21, B0YJB8, E9PPM2
UniProt Related Accession:	O75604
Molecular Weight:	605
NCBI Full Name:	Ubiquitin carboxyl-terminal hydrolase 2
NCBI Synonym Full Names:	ubiquitin specific peptidase 2
NCBI Official Symbol:	USP2
NCBI Official Synonym Symbols:	USP9; UBP41
NCBI Protein Information:	ubiquitin carboxyl-terminal hydrolase 2; ubiquitin thioesterase 2; deubiquitinating enzyme 2; ubiquitin specific protease 9; ubiquitin specific protease 12; 41 kDa ubiquitin-specific protease; ubiquitin-specific-processing protease 2
UniProt Protein Name:	Ubiquitin carboxyl-terminal hydrolase 2
UniProt Synonym Protein Names:	41 kDa ubiquitin-specific protease; Deubiquitinating enzyme 2; Ubiquitin thioesterase 2; Ubiquitin-specific-processing protease 2
Protein Family:	Protein
UniProt Gene Name:	USP2
UniProt Entry Name:	UBP2_HUMAN