UBR3 Antibody (PACO60168)
- SKU:
- PACO60168
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- IHC
- Applications:
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | UBR3 Antibody (PACO60168) |
| Antibody SKU: | PACO60168 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:500-1:1000, IF:1:200-1:500 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human E3 ubiquitin-protein ligase UBR3 protein (960-1113AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | IHC image of PACO60168 diluted at 1:600 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | Immunofluorescence staining of Hela cells with PACO60168 at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
 | IHC image of PACO60168 diluted at 1:600 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| Background: | E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Does not bind to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. |
| Synonyms: | E3 ubiquitin-protein ligase UBR3 (EC 2.3.2.27) (N-recognin-3) (RING-type E3 ubiquitin transferase UBR3) (Ubiquitin-protein ligase E3-alpha-3) (Ubiquitin-protein ligase E3-alpha-III) (Zinc finger protein 650), UBR3, KIAA2024 ZNF650 |
| UniProt Protein Function: | UBR3: E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Does not bind to proteins bearing specific N- terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. Belongs to the UBR1 family. 4 isoforms of the human protein are produced by alternative splicing.Protein type: Ligase; EC 6.3.2.19; Ubiquitin ligase; Membrane protein, integral; Membrane protein, multi-pass; Ubiquitin conjugating system; EC 6.3.2.-Chromosomal Location of Human Ortholog: 2q31.1Cellular Component: cytoplasm; ubiquitin ligase complexMolecular Function: ubiquitin-protein ligase activityBiological Process: embryonic development; sensory perception of smell; suckling behavior; ubiquitin-dependent protein catabolic process |
| UniProt Protein Details: | |
| NCBI Summary: | |
| UniProt Code: | Q6ZT12 |
| NCBI GenInfo Identifier: | 115527310 |
| NCBI Gene ID: | 130507 |
| NCBI Accession: | BC113010 |
| UniProt Secondary Accession: | Q6ZT12,Q2KHN5, Q6ZR55, Q6ZSC2, Q8IVE7, Q8ND96, B4DZR7 |
| UniProt Related Accession: | Q6ZT12 |
| Molecular Weight: | 215,971 Da |
| NCBI Full Name: | Homo sapiens cDNA clone IMAGE:40036320, containing frame-shift errors |
| NCBI Synonym Full Names: | ubiquitin protein ligase E3 component n-recognin 3 (putative) |
| NCBI Official Symbol: | UBR3 |
| NCBI Official Synonym Symbols: | ZNF650 |
| NCBI Protein Information: | E3 ubiquitin-protein ligase UBR3 |
| UniProt Protein Name: | E3 ubiquitin-protein ligase UBR3 |
| UniProt Synonym Protein Names: | N-recognin-3; Ubiquitin-protein ligase E3-alpha-3; Ubiquitin-protein ligase E3-alpha-III; Zinc finger protein 650 |
| Protein Family: | E3 ubiquitin-protein ligase |
| UniProt Gene Name: | UBR3 |
| UniProt Entry Name: | UBR3_HUMAN |
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