Product Name:	Total Protein (Urine/CSF) Assay Kit (BA0179)
SKU:	BA0179
Detection Method:	Colorimetric (OD600 nm)
Detection Range:	5 - 200 mg/dL protein; 1 - 20 mg/dL protein for urine samples
Sample Type:	Urine and cerebrospinal fluid
Species Reactivity:	All
Assay Time:	10 min
Kit Size:	100 Assays
Equipment Required:	Microplate reader
Storage:	2-8C
Shelf Life:	12 months after receipt
Shipping:	Room Temperature

A rapid colorimetric assay for the quantitative determination of total protein based on an improved pyrogallol red-molybdate dye-binding method. The colour intensity measured at 600 nm is directly proportional to the total protein concentration, with a linear range of 5-200 mg/dL (1-20 mg/dL for urine samples using an internal standard). The single reagent is suited to determining protein in urine and cerebrospinal fluid, with minimal interference (< 2%) from urine substances.

Fast and sensitive; assay takes 10 minutes with a linear range of 5 - 200 mg/dL protein (1 - 20 mg/dL for urine)
Robust and reliable; substances in urine have minimal interference (< 2%)
Convenient homogeneous mix-incubate-measure format
Readily automated on high-throughput liquid-handling systems

Determination of total protein in urine (proteinuria)
Determination of total protein in cerebrospinal fluid (blood-brain barrier assessment)

Note: The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.

Step	Procedure
1	Bring all reagents to room temperature. Standard procedure: pipette 5 uL dH2O, 5 uL provided Protein Standard and 5 uL samples into separate wells of a clear flat-bottom 96-well plate.
2	Add 200 uL Reagent to all assay wells and tap the plate to mix.
3	Incubate for 10 minutes and measure OD600nm on a plate reader.
4	Urine samples: dilute the 100 mg/dL Standard to 40 mg/dL, run samples in duplicate, and transfer 20 uL of each sample into two Sample wells and two Internal Standard wells; add 5 uL dH2O to Sample wells and 5 uL of the 40 mg/dL Standard to Internal Standard wells, with 25 uL dH2O as the Blank in duplicate.
5	For urine, add 200 uL Reagent to each protein-determination well, incubate 10 minutes at room temperature and read OD at 600 nm.

Standard samples: [Protein] = [(OD_STANDARD - OD_H2O) / (OD_SAMPLE - OD_H2O)] x 100 x n (mg/dL). Internal-standard samples (e.g. urine): [Protein] = [(OD_STANDARD - OD_SAMPLE) / (OD_SAMPLE - OD_BLANK)] x 10 x n (mg/dL). If the calculated protein concentration exceeds 200 mg/dL, dilute the sample in water and repeat the assay, multiplying the result by the dilution factor n.

Component	Quantity	Storage
Reagent	20 mL	2-8C
Standard (100 mg/dL)	1 mL	2-8C

Neuroimmunologie: Das Immunsystem des ZNS

Interleukin-8-Signalisierung

Waardenburg-Syndrom und Klein-Waardenburg-Syndrom

Cell Biology Assays

Total Protein (Urine/CSF) Assay Kit (BA0179)

Description