Cell Biology Assays

Total Protein (Urine/CSF) Assay Kit (BA0179)

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SKU:
BA0179
Product Type:
Assay
Instrument:
Microplate Reader
Sample Type:
Urine, Cerebrospinal Fluid
Research Area:
Clinical Chemistry

Description

Description

Assay Genie's Total Protein Assay Kit is based on an improved pyrogallol red-molybdate protein dye-binding assay. The color intensity at 600nm is directly proportional to the total protein concentration in the sample. This single reagent can be used for the quantitative determination of total protein in urine (proteinuria) and cerebrospinal fluid (blood brain barrier).


Applications

For quantitative determination of total protein.


Key Features

  • Fast and sensitive. Assay takes 10 min. Linear detection range of 5 - 200 mg/dL protein.
  • Robust and Reliable. Substances in urine have minimal interference (< 2%).
  • Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Key Information Description
Kit Includes
Reagent 20 mL, Standard 1 mL 100 mg/dL
Kit Requires
For 96-well plate assays: pipetting devices, clear flat bottom 96-well plates, and plate reader. For cuvette assays: pipetting devices, cuvettes and spectrophotometer capable of reading OD600nm.
Method of Detection
OD600nm (Pyrogallol Red)
Detection Limit
5 mg/dL
Samples
Urine, cerebrospinal fluid etc
Species
All
Protocol Length
10 min
Size
100 tests
Storage
Store kit at 2-8°C.
Shelf Life
6 months


More Details

Total protein concentration can be determined by several methods. However, protein dye-binding procedures are simple, reliable, rapid, readily automated and are becoming increasingly popular in assessing renal and blood brain barrier functions.

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