Cell Biology Assays

Total Protein Assay Kit (BA0174)

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SKU:
BA0174
Product Type:
Assay
Instrument:
Microplate Reader
Sample Type:
Biological Samples
Research Area:
Amino Acid and Protein

Description

ELISA Kit Technical ManualMSDS

Total Protein Assay Kit - Information

Assay Genie's fluorometric protein assay kit is based on an improved o-phthalaldehyde method. This reagent reacts with primary amines in protein or peptide and forms a blue fluorescent product, allowing detection of nanograms of proteins. The fluorescence intensity (Ex/Em = 360/450nm) is proportional to the protein concentration in the sample.

Applications

For quantitative determination of total protein

Total Protein Assay Kit -Key Features

  • Fast and sensitive. Assay is completed within a few minutes. Linear detection range of 0.05 - 200 ug/mL BSA.
  • Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Total Protein Assay Kit - Data Sheet

Kit IncludesReagent: 20 mL Standard: 1 mL 1mg/mL BSA
Kit RequiresPipetting devices, centrifuge tubes, black flat bottom 96-well plates and plate reader
Method of DetectionFL360/450nm
Detection Limit50 ng/mL
SamplesBiological
SpeciesAll
Protocol Length10 min
Size200 tests
StorageFor long-term storage, keep kit at -20°C
Shelf Life6 months

More Details

The protein or peptide is known as the "building blocks of life" and is one of the most important macromolecules in life science. Protein determination is a very common practice. Simple, direct and automation-ready procedures for measuring protein or peptide concentration are very desirable.
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