Cell Biology Recombinant Proteins
TGM3/Transglutaminase 3 Recombinant Protein (RPES4730)
- SKU:
- RPES4730
Description
TGM3/Transglutaminase 3 Recombinant Protein
Transglutaminases (TGase) are a family of calcium-dependent acyl-transfer enzymes ubiquitously expressed in mammalian cells and responsible for catalyzing covalent cross-links between proteins or peptides. Transglutaminase 3 (TGM3) is a member of a family of Ca2+-dependent enzymes that catalyze covalent cross-linking reactions between proteins or peptides. TGM3 isoform is widely expressed and is important for epithelial barrier formation. It is a zymogen, requiring proteolysis for activity. Calcium-activated TGM3 can bind, hydrolyze, and is inhibited by GTP, despite lacking structural homology with other GTP binding proteins. TGM3 displays a diffuse cytoplasmic distribution in vitro consistent with its proposed role in the early phase of cornified cell envelope assembly in the cytoplasm. TGM3-driven specific isopeptide bonds between intermediate filaments and KAPs participate to the progressive scaffolding of the hair shaft. Additionally, TGM3 may be a novel prognostic biomarker for esophageal squamous cell carcinoma (ESCC).
| Product Name: | TGM3/Transglutaminase 3 Recombinant Protein (RPES4730) |
| Product Code: | RPES4730 |
| Size: | 50µg |
| Species: | Human |
| Expressed Host: | Baculovirus-Insect Cells |
| Synonyms: | TGE |
| Accession: | Q08188 |
| Sequence: | Ala 2-Glu 693 |
| Fusion tag: | N-His |
| Activity: | Measured by its ability to cleave a synthetic peptide Benzyloxycarbonyl-Gln-Gly and NH2OH.The specific activity is > 450 pmoles/min/µg. |
| Endotoxin: | <1.0 EU per µg as determined by the LAL method. |
| Protein Construction: | A DNA sequence encoding the human TGM3 (Q08188) (Ala 2-Glu 693) was expressed, with a polyhistidine tag at the N-terminus. |
| Purity: | > 95 % as determined by reducing SDS-PAGE. |
| Mol Mass: | 78.8 kDa |
| AP Mol Mass: | 70 kDa |
| Formulation: | Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 8.5, 10% gly |
| Shipping: | This product is provided as lyophilized powder which is shipped with ice packs. |
| Stability and Storage: | Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
Additional Information
Product Type: |
Recombinant Protein |
Species: |
Human |
Research Area: |
Cell Biology |
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