Sucrose Assay Kit - Fluorometric (MAES0013)
- SKU:
- MAES0013
- Product Type:
- Assay
- Detection Method:
- Fluorometric
- Instrument:
- Microplate Reader
- Sample Type:
- Plant Tissue
- Sensitivity:
- 0.15 umol/L
- Range:
- 0.15-15 umol/L
- Assay Time:
- 70 min
- Shipping:
- Gel Pack
- Research Area:
- Diabetes & Obesity
- Research Area:
- Food Safety & Analysis
- Research Area:
- Clinical Chemistry
Description
| Product Name: | Sucrose Assay Kit - Fluorometric |
| Product Code: | MAES0013 |
| Product Size: | 96 Assays |
| Sample Type: | Plant tissue |
| Assay Time: | 70 min |
| Instrument: | Fluorescence microplate reader |
| Sensitivity: | 0.15 µmol/L |
| Detection Range: | 0.15-15 µmol/L |
| Recovery Rate: | 96 |
| Inter CV: | 6.5 |
| Inter CV: | 2.3 |
Sucrose, a kind of disaccharide, is a major component of sugar. It is widely found in the leaves, flowers, stems, seeds and fruits of plants. It is an important product of plant photosynthesis and a major form of storage, accumulation and transportation of sugar in plants. Sucrose biosynthesis is catalyzed by sucrose phosphate synthase and 6 '-sucrose phosphate phosphatase and can through glycolysis and tricarboxylic acid cycle to produce ATP and NADH. Sucrose is particularly rich in sugarcane, sugar beet and other sugar crops and fruits. It is an important condiment of food and sweet.
Sucrose can be hydrolyzed by sucrase to produce glucose under acidic conditions, which is catalyzed by glucose oxidase to produce hydrogen peroxide. In the presence of HRP (horse radish peroxidase), hydrogen peroxide reacts with the fluorescent probe to form red fluorescent substance. The sucrose content can be calculated by measuring the fluorescence intensity at the excitation wavelength of 535 nm and the emission wavelength of 590 nm.
| Shelf Life: | 6 Months |
| Storage: | -20°C |
| Shipping: | Gel Pack |
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