Name: SOX3 Antibody (PACO56988)
SKU: PACO56988
Availability: OutOfStock

Antibody Name:	SOX3 Antibody (PACO56988)
Antibody SKU:	PACO56988
Size:	50ug
Host Species:	Rabbit
Tested Applications:	ELISA, WB, IHC
Recommended Dilutions:	ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:200-1:500
Species Reactivity:	Human
Immunogen:	Recombinant Human Transcription factor SOX-3 protein (4-118AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

Western Blot. Positive WB detected in: MCF-7 whole cell lysate. All lanes: SOX3 antibody at 8.6µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 46 kDa. Observed band size: 46 kDa.

IHC image of PACO56988 diluted at 1:300 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Background:

Transcription factor required during the formation of the hypothalamo-pituitary axis. May function as a switch in neuronal development. Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation. Required also within the pharyngeal epithelia for craniofacial morphogenesis. Controls a genetic switch in male development. Is necessary for initiating male sex determination by directing the development of supporting cell precursors (pre-Sertoli cells) as Sertoli rather than granulosa cells (By similarity).

Synonyms:

Transcription factor SOX-3, SOX3

UniProt Protein Function:	SOX3: Transcription factor required during the formation of the hypothalamo-pituitary axis. May function as a switch in neuronal development. Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation. Required also within the pharyngeal epithelia for craniofacial morphogenesis. Controls a genetic switch in male development. Is necessary for initiating male sex determination by directing the development of supporting cell precursors (pre-Sertoli cells) as Sertoli rather than granulosa cells. Defects in SOX3 are a cause of panhypopituitarism X- linked (PHPX). Affected individuals have absent infundibulum, anterior pituitary hypoplasia, and ectopic posterior pituitary. Defects in SOX3 are the cause of mental retardation X- linked with isolated growth hormone deficiency (MRXGH). Defects in SOX3 are the cause of 46,XX sex reversal type 3 (SRXX3). A condition in which male gonads develop in a genetic female (female to male sex reversal). Copy number variations (CNV) encompassing or in close proximity to SOX3 are responsible for XX male reversal. These variations include two duplications of approximately 123 kb and 85 kb, the former of which spans the entire SOX3 gene; a 343 kb deletion immediately upstream of SOX3 that is probably responsible of altered regulation (and not increased dosage) of SOX3; a large (approximately 6 Mb) duplication that encompasses SOX3 and at least 18 additional distally located genes. Its proximal breakpoint falls within the SOX3 regulatory region. This large rearrangement has been found in a patient with XX male reversal and a complex phenotype that also includes a scrotal hypoplasia, microcephaly, developmental delay, and growth retardation.
UniProt Protein Details:	Protein type:DNA-binding; Nuclear receptor co-regulator; Transcription factor Chromosomal Location of Human Ortholog: Xq27.1 Cellular Component: nucleoplasm Molecular Function:DNA binding Biological Process: central nervous system development; hypothalamus development; negative regulation of neuron differentiation; pituitary gland development; sensory organ development; sex determination Disease: Mental Retardation, X-linked, With Panhypopituitarism; Panhypopituitarism, X-linked
NCBI Summary:	This gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins. Mutations in this gene have been associated with X-linked mental retardation with growth hormone deficiency. [provided by RefSeq, Jul 2008]
UniProt Code:	P41225
NCBI GenInfo Identifier:	48429228
NCBI Gene ID:	6658
NCBI Accession:	P41225.2
UniProt Secondary Accession:	P41225,P35714, Q5JWI3, Q9NP49,
UniProt Related Accession:	P41225
Molecular Weight:	45,210 Da
NCBI Full Name:	Transcription factor SOX-3
NCBI Synonym Full Names:	SRY-box 3
NCBI Official Symbol:	SOX3
NCBI Official Synonym Symbols:	PHP; GHDX; MRGH; PHPX; SOXB
NCBI Protein Information:	transcription factor SOX-3
UniProt Protein Name:	Transcription factor SOX-3
Protein Family:	Transcription factor
UniProt Gene Name:	SOX3
UniProt Entry Name:	SOX3_HUMAN