The SMMHC/MYH11 Monoclonal Antibody (CAB22339) is a high-quality antibody developed for reliable detection and analysis of target proteins. This monoclonal antibody, produced through hybridoma technology, is highly specific to human SMMHC and is ideal for a variety of research applications, including Western blotting and immunohistochemistry.SMMHC, encoded by the MYH11 gene, is a key component of smooth muscle cells and is essential for their contractile function. Dysregulation of SMMHC has been implicated in a variety of smooth muscle-related diseases, including vascular disorders and various gastrointestinal conditions.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
SMMHC/MYH11 Monoclonal Antibody
SKU:
CAB22339
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC51916
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
The protein encoded by this gene is a smooth muscle myosin belonging to the myosin heavy chain family. The gene product is a subunit of a hexameric protein that consists of two heavy chain subunits and two pairs of non-identical light chain subunits. It functions as a major contractile protein, converting chemical energy into mechanical energy through the hydrolysis of ATP. A chromosomal rearrangement involving this gene is associated with acute myeloid leukemia of the M4Eo subtype. Mutations in this gene are associated with visceral myopathy, megacystis-microcolon-intestinal hypoperistalsis syndrome 2, and familial thoracic aortic aneurysm 4.
Purification Method
Affinity purification
Gene ID
4629
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using SMMHC/MYH11 Rabbit mAb (CAB22339) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Western blot analysis of lysates from A375 cells, using SMMHC/MYH11 Rabbit mAb (CAB22339) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
