SKA1 Antibody (PACO57372)
- SKU:
- PACO57372
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- IHC
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | SKA1 Antibody (PACO57372) |
| Antibody SKU: | PACO57372 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, IHC |
| Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:200-1:500 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Spindle and kinetochore-associated protein 1 protein (61-134AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | IHC image of PACO57372 diluted at 1:200 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| Background: | Component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. Required for timely anaphase onset during mitosis, when chromosomes undergo bipolar attachment on spindle microtubules leading to silencing of the spindle checkpoint. The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies. The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner. Affinity for microtubules is synergistically enhanced in the presence of the ndc-80 complex and may allow the ndc-80 complex to track depolymerizing microtubules. In the complex, it mediates the interaction with microtubules. |
| Synonyms: | Spindle and kinetochore-associated protein 1, SKA1, C18orf24 |
| UniProt Protein Function: | SKA1: Component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. Required for timely anaphase onset during mitosis, when chromosomes undergo bipolar attachment on spindle microtubules leading to silencing of the spindle checkpoint. The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies. The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner. In the complex, it mediates the interaction with microtubules. Belongs to the SKA1 family. 2 isoforms of the human protein are produced by alternative splicing.Protein type: Unknown functionChromosomal Location of Human Ortholog: 18q21.1Cellular Component: microtubule cytoskeleton; spindle microtubule; outer kinetochore of condensed chromosome; cytosolMolecular Function: protein binding; microtubule bindingBiological Process: mitosis; cell division; mitotic cell cycle; regulation of microtubule polymerization or depolymerization; chromosome segregation |
| UniProt Protein Details: | |
| NCBI Summary: | |
| UniProt Code: | Q96BD8 |
| NCBI GenInfo Identifier: | 74731226 |
| NCBI Gene ID: | 220134 |
| NCBI Accession: | Q96BD8.1 |
| UniProt Secondary Accession: | Q96BD8,B2R9Y6, B4E0P4 |
| UniProt Related Accession: | Q96BD8 |
| Molecular Weight: | 24,390 Da |
| NCBI Full Name: | Spindle and kinetochore-associated protein 1 |
| NCBI Synonym Full Names: | spindle and kinetochore associated complex subunit 1 |
| NCBI Official Symbol: | SKA1 |
| NCBI Official Synonym Symbols: | C18orf24 |
| NCBI Protein Information: | spindle and kinetochore-associated protein 1; spindle and KT (kinetochore) associated 1 |
| UniProt Protein Name: | Spindle and kinetochore-associated protein 1 |
| UniProt Synonym Protein Names: | |
| Protein Family: | Spindle and kinetochore-associated protein |
| UniProt Gene Name: | SKA1 |
| UniProt Entry Name: | SKA1_HUMAN |
Related Products
SPEF2 Antibody
Overview Product Name:SPEF2 AntibodyProduct Code:PACO64977Size:50µLTarget Names:SPEF2Species Reactivity:HumanHos
ompF Antibody
Overview Product Name:ompF AntibodyProduct Code:PACO64865Size:50µLTarget Names:ompFSpecies Reactivity:Escherichia coli
Hmgb1 Antibody
Overview Product Name:Hmgb1 AntibodyProduct Code:PACO64769Size:50µLTarget Names:Hmgb1Species Reactivity:RatHost
RIMS4 Antibody
Overview Product Name:RIMS4 AntibodyProduct Code:PACO64925Size:50µLTarget Names:RIMS4Species Reactivity:HumanHos