Human SKA1 Recombinant Protein (RPPB4712)

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RPPB4712
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Description

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Product Name:Human SKA1 Recombinant Protein
Product Code:RPPB4712
Size:5µg
Species:Human
Target:SKA1
Synonyms:Spindle and kinetochore-associated protein 1, SKA1, C18orf24, Spindle And Kinetochore Associated Complex Subunit 1.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The SKA1 solution (0.25mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 10% glycerol and 0.1M NaCl.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 80.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSMASSDLE QLCSHVNEKI GNIKKTLSLR NCGQEPTLKT VLNKIGDEII VINELLNKLE LEIQYQEQTN NSLKELCESL EEDYKDIEHL KENVPSHLPQ VTVTQSCVKG SDLDPEEPIK VEEPEPVKKP PKEQRSIKEM PFITCDEFNG VPSYMKSRLT YNQINDVIKE INKAVISKYK ILHQPKKSMN SVTRNLYHRF IDEETKDTKG RYFIVEADIK EFTTLKADKK FHVLLNILRH CRRLSEVRGG GLTRYVIT

Spindle And Kinetochore Associated Complex Subunit 1 (SKA1) is a part of the kinetochore-microtubule interface which associates with microtubules as oligomeric assemblies. The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner. SKA1 is necessary for timely anaphase onset during mitosis, when chromosomes undergo bipolar attachment on spindle microtubules leading to silencing of the spindle checkpoint.

SKA1 Human Recombinant produced in E. coli is a single polypeptide chain containing 278 amino acids (1-255) and having a molecular mass of 31.9 kDa.SKA1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:SKA1: Component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. Required for timely anaphase onset during mitosis, when chromosomes undergo bipolar attachment on spindle microtubules leading to silencing of the spindle checkpoint. The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies. The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner. In the complex, it mediates the interaction with microtubules. Belongs to the SKA1 family. 2 isoforms of the human protein are produced by alternative splicing.Protein type: Unknown functionChromosomal Location of Human Ortholog: 18q21.1Cellular Component: microtubule cytoskeleton; spindle microtubule; outer kinetochore of condensed chromosome; cytosolMolecular Function: protein binding; microtubule bindingBiological Process: mitosis; cell division; mitotic cell cycle; regulation of microtubule polymerization or depolymerization; chromosome segregation
UniProt Protein Details:
NCBI Summary:
UniProt Code:Q96BD8
NCBI GenInfo Identifier:74731226
NCBI Gene ID:220134
NCBI Accession:Q96BD8.1
UniProt Secondary Accession:Q96BD8,B2R9Y6, B4E0P4
UniProt Related Accession:Q96BD8
Molecular Weight:24,390 Da
NCBI Full Name:Spindle and kinetochore-associated protein 1
NCBI Synonym Full Names:spindle and kinetochore associated complex subunit 1
NCBI Official Symbol:SKA1��
NCBI Official Synonym Symbols:C18orf24��
NCBI Protein Information:spindle and kinetochore-associated protein 1; spindle and KT (kinetochore) associated 1
UniProt Protein Name:Spindle and kinetochore-associated protein 1
UniProt Synonym Protein Names:
Protein Family:Spindle and kinetochore-associated protein
UniProt Gene Name:SKA1��
UniProt Entry Name:SKA1_HUMAN
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Additional Information

Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q96BD8
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