Sheep ELISA Kits
Sheep VWF (Von Willebrand Factor) ELISA Kit (SHFI00039)
- SKU:
- SHFI00039
- Product Type:
- ELISA Kit
- Uniprot:
- W5PG63
- ELISA Type:
- Sandwich
- Synonyms:
- Von Willebrand Factor
- Reactivity:
- Sheep
- Size:
- 48T/96T
- Detection Method:
- Sandwich ELISA, Double Antibody
- Sensitivity:
- 0.938 pg/mL
- Range:
- 1.563-100 pg/mL
Description
Sheep VWF (Von Willebrand Factor) ELISA Kit
The Sheep VWF (Von Willebrand Factor) ELISA Kit is specifically designed for the accurate and precise measurement of VWF levels in sheep serum, plasma, and other biological samples. This kit offers high sensitivity and specificity, providing researchers with reliable and reproducible results for a variety of research applications. VWF is a key protein involved in blood clotting and plays a crucial role in hemostasis. Dysregulation of VWF levels can lead to bleeding disorders such as Von Willebrand Disease.
By accurately measuring VWF levels, researchers can gain valuable insights into the pathophysiology of bleeding disorders and cardiovascular diseases in sheep. Overall, the Sheep VWF ELISA Kit is a valuable tool for researchers studying hemostasis and thrombosis in sheep, offering a reliable and efficient means of quantifying VWF levels in biological samples.
| Product Name: | Sheep VWF (Von Willebrand Factor) ELISA Kit |
| SKU: | SHFI00039 |
| Reactivity: | Sheep |
| Assay Type: | Sandwich ELISA, Double Antibody |
| Sensitivity: | 0.938 pg/mL |
| Range: | 1.563-100 pg/mL |
| Sample Type: | Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples |
| Storage: | 2-8°C for 12 months. |
| Linearity: |
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| Recovery: |
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Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
| Step | Procedure |
| 1 | Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions. |
| 2 | Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding. |
| 3 | Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes. |
| 4 | HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes. |
| 5 | Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes. |
| 6 | Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately. |
| Sample Type | Protocol |
| Serum | Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately. |
| Plasma | Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma. |
| Tissue Homogenate | Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant. |
| Cell Culture Supernatant | Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant. |
| Cell Lysate | Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant. |
| Other Sample Types | For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com. |
| Component | Quantity | Storage | |
| 48T | 96T | ||
| ELISA Microplate (Dismountable) | 8×6 | 8×12 | Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C. |
| Lyophilized Standard | 1 vial | 2 vial | Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C. |
| Biotin-labeled Antibody (Concentrated, 100X) | 60 ul | 120 ul | 2-8°C (Avoid direct light) |
| HRP-Streptavidin Conjugate (SABC, 100X) | 60 ul | 120 ul | 2-8°C (Avoid direct light) |
| TMB Substrate | 5 ml | 10 ml | 2-8°C (Avoid direct light) |
| Sample Dilution Buffer | 10 ml | 20 ml | 2-8°C |
| Antibody Dilution Buffer | 5 ml | 10 ml | 2-8°C |
| SABC Dilution Buffer | 5 ml | 10 ml | 2-8°C |
| Stop Solution | 5 ml | 10 ml | 2-8°C |
| Wash Buffer(25X) | 15 ml | 30 ml | 2-8°C |
| Plate Sealer | 3 pieces | 5 pieces | - |
| Technical Manual | 1 copy | 1 copy | - |
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