The RGL2 Polyclonal Antibody (CAB22119) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, demonstrates high reactivity with human samples and is validated for use in Western blot applications.RGL2, also known as Ral guanine nucleotide dissociation stimulator-like 2, plays a crucial role in regulating cell growth and survival. Its involvement in signaling pathways makes it an important target for research in cancer biology and developmental biology. By binding to the RGL2 protein, this antibody allows for the detection and analysis of RGL2 expression in different cell types, providing valuable insights into its functions.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Arabidopsis thaliana samples.
Product Name:
RGL2 Polyclonal Antibody
SKU:
CAB22119
Size:
20μL, 100μL
Reactivity:
Arabidopsis thaliana
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
RGA-like 2, T21P5.13, T21P5_13, RGL2
Positive Sample:
Seedling, Inflorescences, Before inflorescence, Rosette leaf
Cellular Localization:
Nucleus.
Calculated MW:
60kDa
Observed MW:
60kDa
Encodes a DELLA protein, a member of the GRAS superfamily of putative transcription factors. DELLA proteins restrain the cell proliferation and expansion that drives plant growth. Negative regulator of the response to GA in controlling seed germination. GA triggers the degradation of RGL2 protein in a process blocked by both proteasome inhibitors and serine/threonine phosphatase inhibitors. The protein undergoes degradation in response to GA via the 26S proteasome. RGL2 may be involved in reducing ROS accumulation in response to stress by up-regulating the transcription of superoxide dismutases. Rapidly degraded in response to GA. Regulates GA-promoted seed germination. Involved in flower and fruit development.
Purification Method
Affinity purification
Gene ID
821251
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various tissues from Arabidopsis thaliana, using RGL2 Rabbit pAb (CAB22119) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates / proteins: 25 μg per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 60s.
