Recombinant Mouse IL-17F Protein

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Protein Description:	Recombinant Mouse IL-17F Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Met1-Ala161) of mouse IL-17F (Accession #NP_665855.2) fused with a 6×His tag at the C-terminus.
Endotoxin:	< 0.1 EU/μg of the protein by LAL method.
Purity:	≥ 95 % as determined by SDS-PAGE;≥ 95 % as determined by HPLC.
Formulation:	Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Gene ID:	257630

Storage:	Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Reconstitution:	Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Swiss-Prot:	NP_665855.2

	Recombinant Mouse IL-17F Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
	Immobilized recombinant Mouse IL-17F at 2μg/mL (100μL/well) can bind recombinant Mouse IL17RA with a linear range of 31. 25-163ng/mL.
	Measured by its ability to induce IL-6 secretion by NIH-3T3 mouse embryonic fibroblast cells in the presense of 2 ng/mL TNFα(Catalog: RPCB0855). The ED50 for this effect is 10. 18-140.72 ng/mL, corresponding to a specific activity of 2. 46×10 4~9. 82×10 4 units/mg.