Rat TRACP-5b (Tartrate-Resistant Acid Phosphatase 5b) ELISA Kit
The Rat TRACP-5B (Tartrate-Resistant Acid Phosphatase 5B) ELISA Kit is specially designed for the precise measurement of TRACP-5B levels in rat serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring dependable and consistent outcomes, making it perfect for a variety of research purposes.TRACP-5B is an enzyme linked to bone metabolism and is essential for osteoclast function. It is a crucial marker for bone resorption and turnover, playing a key role in bone-related disorders such as osteoporosis and osteoarthritis. The Rat TRACP-5B ELISA Kit is an invaluable tool for investigating bone health and disease progression, providing valuable insights for potential therapeutic interventions.
Product Name:
Rat TRACP-5b (Tartrate-Resistant Acid Phosphatase 5b) ELISA Kit
SKU:
RTES00780
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
