The Rat SIGA (Secretory Immunoglobulin A) ELISA Kit is specifically designed for the quantitative measurement of secretory immunoglobulin A levels in rat samples, including serum, plasma, and cell culture supernatants. This kit offers excellent sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.Secretory immunoglobulin A (SIGA) is an important component of the immune system, playing a key role in protecting mucosal surfaces from pathogens and allergens. Studies have shown that alterations in SIGA levels are associated with various inflammatory and infectious diseases, making it a valuable biomarker for immunological research and diagnostics.Overall, the Rat SIGA ELISA Kit provides researchers with a reliable tool for studying the role of secretory immunoglobulin A in immune responses and disease pathogenesis, ultimately aiding in the development of novel therapeutic strategies.
Product Name:
Rat sIgA (Secretory Immunoglobulin A) ELISA Kit
SKU:
RTES00729
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
