Rat Immunology ELISA Kits 2

Rat HO-1/HMOX1/HSP32 ELISA Kit (RTFI00859)

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SKU:
RTFI00859
Product Type:
ELISA Kit
Uniprot:
P06762
ELISA Type:
Sandwich
Synonyms:
HO-1, HO1, HMOX1, HSP32, heat shock protein, 32-kD, heme oxygenase, decycling 1, HO, HO1
Reactivity:
Rat
Research Area:
Cell Death
Size:
48T/96T
Detection Method:
Sandwich ELISA, Double Antibody
Sensitivity:
0.188 ng/mL
Range:
0.313-20 ng/mL
Frequently bought together:

Description

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Rat HO-1/HMOX1/HSP32 ELISA Kit

The Rat HO-1 (Hmox1/HSP32) ELISA Kit from Assay Genie is specifically designed for the precise measurement of Hmox1/HSP32 levels in rat serum, plasma, and tissue lysate samples. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications. Hmox1/HSP32, also known as heme oxygenase-1, is a critical enzyme involved in heme catabolism and has been linked to various physiological and pathological processes.

Its regulatory role in oxidative stress, inflammation, and cell signaling makes it a valuable biomarker for studying conditions such as cardiovascular diseases, diabetes, and neurodegenerative disorders, providing insights for potential therapeutic interventions. Order your Rat HO-1 (Hmox1/HSP32) ELISA Kit from Assay Genie today and unlock the potential for groundbreaking research in the field of oxidative stress and disease pathology.

Product Name:Rat HO-1/HMOX1/HSP32 ELISA Kit
SKU:RTFI00859
Reactivity:Rat
Assay Type:Sandwich ELISA, Double Antibody
Sensitivity:0.188 ng/mL
Range:0.313-20 ng/mL
Sample Type:Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:2-8°C for 12 months.
Linearity:
Sample1:21:41:8
Serum (n = 5)91-105%95-100%85-102%
EDTA Plasma (n = 5)85-97%84-101%82-96%
Heparin Plasma (n = 5)80-95%81-100%81-97%
Recovery:
SampleRecovery Range (%)Average (%)
Serum (n = 5)86-10494
EDTA Plasma (n = 5)89-10193
Heparin Plasma (n = 5)88-10596
Assay Procedure

Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.

StepProcedure
1Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample TypeProtocol
SerumAllow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
PlasmaCollect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue HomogenateHomogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture SupernatantCentrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell LysateLyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample TypesFor more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
ComponentQuantityStorage
48T96T
ELISA Microplate (Dismountable)8×68×12Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard1 vial2 vialPlace the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Biotin-labeled Antibody (Concentrated, 100X)60 ul120 ul2-8°C (Avoid direct light)
HRP-Streptavidin Conjugate (SABC, 100X)60 ul120 ul2-8°C (Avoid direct light)
TMB Substrate5 ml10 ml2-8°C (Avoid direct light)
Sample Dilution Buffer10 ml20 ml2-8°C
Antibody Dilution Buffer5 ml10 ml2-8°C
SABC Dilution Buffer5 ml10 ml2-8°C
Stop Solution5 ml10 ml2-8°C
Wash Buffer(25X)15 ml30 ml2-8°C
Plate Sealer3 pieces5 pieces-
Technical Manual1 copy1 copy-
Aziz et al.Cilostazol protects against cyclophosphamide-induced ovarian toxicity in female rats: role of cAMP and HO-1Toxicology Mechanisms and Methods 2020View Citation
Aziz et al.Fenofbrate ameliorates testicular damage in rats with streptozotocin‑induced type 1 diabetes:role of HO‑1 and p38 MAPKPharmacological Reports 2020PMID: 32515004
Waz et al.Cardioprotective effect of allyl isothiocyanate in a rat model of doxorubicin acute toxicityToxicology Mechanisms and Methods 2021PMID: 34635025
Kamel et al.Role of IL-6/STAT3 pathway in mediating the protective effect of agomelatine against methotrexate-induced lung/intestinal tissues damage in ratsImmunopharmacology and Immunotoxicology 2021PMID: 34766527
Khalaf et al.Role of Platelet-activating factor and HO-1 in mediating the protective effect of rupatadine against 5-fluorouracil-induced hepatotoxicity in ratsEnvironmental Science and Pollution Research 2022PubMed ID: 35119631
Khalaf et al.Rivastigmine ameliorates indomethacin experimentally induced gastric mucosal injury via activating α7nAChR with inhibiting oxidative stress and apoptosisJournal of Biochemical and Molecular Toxicology 2022PubMed ID: 35702939
Rania H. Abu-Baih et al.Activation of SIRT1/Nrf2/HO-1 and Beclin-1/AMPK/mTOR autophagy pathways by eprosartan ameliorates testicular dysfunction induced by testicular torsion in ratsScientific Reports 2024PubMed ID: 38822026
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