Rat Immunology ELISA Kits 2
Rat BMP6 ELISA Kit (RTFI00602)
- SKU:
- RTFI00602
- Product Type:
- ELISA Kit
- Uniprot:
- Q04906
- ELISA Type:
- Sandwich
- Synonyms:
- BMP-6, BMP6, VGR, VGR1, Vgr-1, bone morphogenetic protein 6, vegetal related growth factor, TGFB-related, vegetal-related, TGFB related cytokine, VG-1-R, VG-1-related protein, Vg1-related sequence
- Reactivity:
- Rat
- Research Area:
- Cell Biology
- Size:
- 48T/96T
- Detection Method:
- Sandwich ELISA, Double Antibody
- Sensitivity:
- 0.094 ng/mL
- Range:
- 0.156-10 ng/mL
Description
Rat BMP6 ELISA Kit
Rat BMP6 ELISA Kit is designed for the accurate and sensitive detection of BMP6 levels in rat serum, plasma, and cell culture supernatants. This kit offers high specificity and reproducibility, ensuring reliable results for various research applications. BMP6, or bone morphogenetic protein 6, is a vital signaling molecule involved in various biological processes, including bone formation, embryonic development, and tissue regeneration.
Dysregulation of BMP6 has been linked to several diseases, such as osteoporosis, cancer, and inflammatory disorders, making it a valuable biomarker for understanding these conditions and exploring potential therapeutic interventions. Overall, the Rat BMP6 ELISA Kit provides a valuable tool for researchers studying BMP6 and its implications in different physiological and pathological processes, offering precise measurements and insights into its role in health and disease.
| Product Name: | Rat BMP6 ELISA Kit |
| SKU: | RTFI00602 |
| Reactivity: | Rat |
| Assay Type: | Sandwich ELISA, Double Antibody |
| Sensitivity: | 0.094 ng/mL |
| Range: | 0.156-10 ng/mL |
| Sample Type: | Serum, Plasma, Cell Culture Supernatant, Cell Lysis Buffer or Tissue Lysis Buffer, and Other Biological Fluid Samples |
| Storage: | 2-8°C for 12 months. |
| Linearity: |
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| Recovery: |
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Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
| Step | Procedure |
| 1 | Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions. |
| 2 | Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding. |
| 3 | Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes. |
| 4 | HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes. |
| 5 | Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes. |
| 6 | Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately. |
| Sample Type | Protocol |
| Serum | Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately. |
| Plasma | Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma. |
| Tissue Homogenate | Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant. |
| Cell Culture Supernatant | Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant. |
| Cell Lysate | Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant. |
| Other Sample Types | For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com. |
| Component | Quantity | Storage | |
| 48T | 96T | ||
| ELISA Microplate (Dismountable) | 8×6 | 8×12 | Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C. |
| Lyophilized Standard | 1 vial | 2 vial | Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C. |
| Biotin-labeled Antibody (Concentrated, 100X) | 60 ul | 120 ul | 2-8°C (Avoid direct light) |
| HRP-Streptavidin Conjugate (SABC, 100X) | 60 ul | 120 ul | 2-8°C (Avoid direct light) |
| TMB Substrate | 5 ml | 10 ml | 2-8°C (Avoid direct light) |
| Sample Dilution Buffer | 10 ml | 20 ml | 2-8°C |
| Antibody Dilution Buffer | 5 ml | 10 ml | 2-8°C |
| SABC Dilution Buffer | 5 ml | 10 ml | 2-8°C |
| Stop Solution | 5 ml | 10 ml | 2-8°C |
| Wash Buffer(25X) | 15 ml | 30 ml | 2-8°C |
| Plate Sealer | 3 pieces | 5 pieces | - |
| Technical Manual | 1 copy | 1 copy | - |
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