QuickStep Rat IL-1R2 (Interleukin 1 Receptor Type II) ELISA Kit
- SKU:
- AEES00664
- Product Type:
- ELISA Kit
- ELISA Type:
- Sandwich
- Size:
- 96 Assays
- Reactivity:
- Rat
- Sensitivity:
- 18.75 pg/mL
- Range:
- 31.25-2000 pg/mL
- Sample Type:
- Serum, plasma and other biological fluids
Description
| Product Name: | QuickStep Rat IL-1R2 (Interleukin 1 Receptor Type II) ELISA Kit |
| Product Code: | AEES00664 |
| Assay Type: | Sandwich |
| Format: | 96 Assays |
| Assay Time: | 1.5h |
| Reactivity: | Rat |
| Detection Range: | 31.25-2000 pg/mL |
| Sensitivity: | 18.75 pg/mL |
| Sample Type & Sample Volume: | Serum, plasma and other biological fluids, 50μL |
| Specificity: | This kit recognizes IL-1R2 in samples. No significant cross-reactivity or interference between IL-1R2 and analogues was observed. |
| Reproducibility: | Both intra-CV and inter-CV are < 10%. |
| Application: | This ELISA kit applies to the in vitro quantitative determination of IL-1R2 concentrations in serum, plasma and other biological fluids. |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IL-1R2. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Rat IL-1R2 are added to the micro ELISA plate wells. Rat IL-1R2 in samples (or standards) combines with the coated antibody and HRP linked detection antibody specific to IL-1R2. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Rat IL-1R2 in the samples is then determined by comparing the OD of the samples to the standard curve.
| Kit Components: | An unopened kit can be stored at 2-8℃ for 6 months. After test, the unused wells and reagents should be stored according to the table.
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