QuickStep Rat IL-1R2 (Interleukin 1 Receptor Type II) ELISA Kit

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SKU:
AEES00664

Description

Product Name:QuickStep Rat IL-1R2 (Interleukin 1 Receptor Type II) ELISA Kit
Product Code:AEES00664
Assay Type:Sandwich
Format:96 Assays
Assay Time:1.5h
Reactivity:Rat
Detection Range:31.25-2000 pg/mL
Sensitivity:18.75 pg/mL
Sample Type & Sample Volume:Serum, plasma and other biological fluids, 50μL
Specificity:This kit recognizes IL-1R2 in samples. No significant cross-reactivity or interference between IL-1R2 and analogues was observed.
Reproducibility:Both intra-CV and inter-CV are < 10%.
Application:This ELISA kit applies to the in vitro quantitative determination of IL-1R2 concentrations in serum, plasma and other biological fluids.

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IL-1R2. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Rat IL-1R2 are added to the micro ELISA plate wells. Rat IL-1R2 in samples (or standards) combines with the coated antibody and HRP linked detection antibody specific to IL-1R2. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Rat IL-1R2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Kit Components:

An unopened kit can be stored at 2-8℃ for 6 months. After test, the unused wells and reagents should be stored according to the table.

ItemSpecificationsStorage
Micro ELISA Plate(Dismountable)8 wells x 12 strips2-8℃, 1 months
Reference Standard2 vials2-8℃, use the reconstituted standard within 24h
Concentrated HRP Linked Detection Ab(100×)1 vial, 60 μL2-8℃(Protect from light)
Reference Standard & Sample Diluent1 vial, 20 mL2-8℃
HRP Linked Ab Diluent1 vial, 14 mL
Concentrated Wash Buffer(25×)96T/: 1 vial, 30 mLL
Substrate Reagent1 vial, 10 mL2-8℃(Protect from light)
Stop Solution1 vial, 10 mL2-8℃
Plate Sealer5 pieces
Product Description1 copy
Certificate of Analysis1 copy
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Additional Information

Product Type:
ELISA Kit
ELISA Type:
Sandwich
Size:
96 Assays
Reactivity:
Rat
Sensitivity:
18.75 pg/mL
Range:
31.25-2000 pg/mL
Sample Type:
Serum, plasma and other biological fluids
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