QuickStep is a revolutionary innovation in pre-coated ELISA kit technologies. Using highly validated antigens & antibodies coupled with optimized buffer systems, QuickStep guarantees super-sensitive quantitative results in 90 minutes with a 1-wash protocol.
This ELISA kit applies to the in vitro quantitative determination of Pg concentrations in serum and plasma.
Specificity:
This kit recognizes Pg in samples. No significant cross-reactivity or interference between Pg and analogues was observed.
Dilution Method:
It is recommended to do the experiment with undiluted Canine serum and plasma samples.
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Storage & Stability:
An unopened kit can be stored at 2-8°C for 6 months. After test, the unused wells and reagents should be stored according to the storage table in the manual.
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Canine E3. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Canine E3 are added to the micro ELISA plate wells. Canine E3 in samples (or standards) competes with a fixed amount of Canine E3 on the solid phase supporter for sites on the HRP linked detection antibody specific to Canine E3. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The concentration of Canine E3 in the samples is then determined by comparing the OD of the samples to the standard curve.
Item
Specifications
Storage
Micro ELISA Plate(Dismountable)
8 wells - 12 strips
2-8°C, 1 months
Reference Standard
2 vials
2-8°C, use the reconstituted standard within 24h
Concentrated HRP Linked Detection Ab(100-)
1 vial, 60 µL
2-8°C(Protect from light)
Reference Standard & Sample Diluent
1 vial, 20 mL
2-8°C
HRP Linked Ab Diluent
1 vial, 14 mL
Concentrated Wash Buffer(25-)
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
2-8°C(Protect from light)
Stop Solution
1 vial, 10 mL
2-8°C
Plate Sealer
5 pieces
Product Description
1 copy
Certificate of Analysis
1 copy
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean (pg/mL)
9.40
24.67
83.80
10.26
23.44
79.70
Standard deviation
0.48
1.06
3.13
0.56
1.13
3.58
CV (%)
5.07
4.28
3.73
5.43
4.83
4.49
Sample Type
Range (%)
Average Recovery (%)
Serum(n=8)
86-101
93
EDTA plasma(n=8)
96-107
101
Samples were spiked with high concentrations of Canine Pg and diluted with Reference Standard & Sample Diluent to produceSamples with values within the range of the assay.
QuickStep Sheep Estriol ELISA KitQuickStep is a revolutionary innovation in pre-coated ELISA kit technologies. Using highly validated antigens & antibodies coupled with optimized buffer systems,...
QuickStep Monkey Estriol ELISA KitQuickStep is a revolutionary innovation in pre-coated ELISA kit technologies. Using highly validated antigens & antibodies coupled with optimized buffer systems,...
QuickStep Rat Estriol ELISA KitQuickStep is a revolutionary innovation in pre-coated ELISA kit technologies. Using highly validated antigens & antibodies coupled with optimized buffer systems,...
QuickStep Human Estriol ELISA KitQuickStep is a revolutionary innovation in pre-coated ELISA kit technologies. Using highly validated antigens & antibodies coupled with optimized buffer systems,...
QuickStep Porcine Estriol ELISA KitQuickStep is a revolutionary innovation in pre-coated ELISA kit technologies. Using highly validated antigens & antibodies coupled with optimized buffer...
QuickStep Mouse Estriol ELISA KitQuickStep is a revolutionary innovation in pre-coated ELISA kit technologies. Using highly validated antigens & antibodies coupled with optimized buffer systems,...
QuickStep Chicken Estriol ELISA KitQuickStep is a revolutionary innovation in pre-coated ELISA kit technologies. Using highly validated antigens & antibodies coupled with optimized buffer...
QuickStep Bovine Estriol ELISA KitQuickStep is a revolutionary innovation in pre-coated ELISA kit technologies. Using highly validated antigens & antibodies coupled with optimized buffer systems,...
