Pyruvate Dehydrogenase (PDH) Activity Assay Kit
- SKU:
- MAES0243
Description
| Product Name: | Pyruvate Dehydrogenase (PDH) Activity Assay Kit |
| Product Code: | MAES0243 |
| Sample Type: | Serum (Plasma), Urine, Animal Tissue, Cell |
| Assay Time: | 15 min |
| Assay Type: | Enzyme Activity |
| Synonyms: | PDH |
| Detection Instrument: | Microplate reader (440-460 nm, optimum wavelength: 450 nm) |
| Sensitivity: | 0.28 U /L |
| Detection Range: | 0.28-76.23 U/L |
| Inter-assay CV: | 0.1 |
| Intra-assay CV: | 0.02 |
Pyruvate dehydrogenase (PDH) catalyzed the oxidative decarylation of pyruvate into acetyl CoA, which plays an important role in the metabolism of mitochondrial respiratory chain and is associated with the aging of cancer genes. Pyruvate can be decarboxylated by PDH under the action of CoA. Meanwhile, NAD+ is reduced to NADH, Which under the action of hydrogen transmitter, transfer electrons to WST-8 to produce the yellow product. The activity of PDH can be calculated by measuring the change of absorbance value at 450 nm.
| Storage: | -20°C |
| Valid Period: | 12 months |
| Other Instruments Required: | Centrifuge, 37℃ incubator |
Additional Information
Product Type: |
Assay |
Instrument: |
Microplate Reader |
Sample Type: |
Serum, Plasma, Urine, Animal Tissue, Cell |
Sensitivity: |
0.28 U /L |
Range: |
0.28-76.23 U/L |
Assay Time: |
15 min |
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