Hormone & Small Molecule ELISA Kits
PREG (Pregnenolone) ELISA Kit (UNFI0110)
- SKU:
- UNFI0110
- Product Type:
- ELISA Kit
- ELISA Type:
- Competitive
- Synonyms:
- Pregnenolone
- Reactivity:
- Universal
- Size:
- 48T/96T
- Detection Method:
- Competitive ELISA, Coated with Antigen
- Sensitivity:
- 9.375 pg/mL
- Range:
- 15.625-1000 pg/mL
Description
PREG (Pregnenolone) ELISA Kit
Pregnenolone ELISA Kit from Assay Genie is a cutting-edge tool designed for the accurate detection of pregnenolone levels in biological samples. This kit offers high sensitivity and specificity, ensuring precise and reliable results for a variety of research applications. Pregnenolone is a key steroid hormone precursor involved in various physiological processes, including hormone production and neuroprotection.
Imbalances in pregnenolone levels have been implicated in conditions such as stress, cognitive decline, and mood disorders, making it a valuable biomarker for investigating these health concerns and exploring potential therapeutic interventions. Overall, the Pregnenolone ELISA Kit from Assay Genie provides researchers with a robust and efficient tool for studying pregnenolone biology and its implications in health and disease.
| Product Name: | PREG (Pregnenolone) ELISA Kit |
| SKU: | UNFI0110 |
| Reactivity: | Universal |
| Assay Type: | Competitive ELISA, Coated with Antigen |
| Sensitivity: | 9.375 pg/mL |
| Range: | 15.625-1000 pg/mL |
| Sample Type: | Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples |
| Storage: | 2-8°C(Sealed), Don't cryopreserve. |
| Linearity: |
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| Recovery: |
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Note: The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
| Step | Procedure |
| 1 | Reagent & Plate Preparation: Equilibrate TMB substrate for 30 minutes at room temperature. Prepare standards, samples (minimum 1:2 dilution), blanks, assign wells, and pre-wash the plate twice. |
| 2 | Sample & Biotin-Antibody Binding: Add 50 µL standard or sample followed by 50 µL biotin-labeled antibody to each well. Mix gently and incubate at 37°C for 45 minutes. |
| 3 | Washing: Wash the plate 5 times with wash buffer, allowing 1 minute soak time per wash. |
| 4 | Color Development: Add TMB substrate and incubate in the dark at 37°C for 10-20 minutes until color develops. |
| 5 | Stop Reaction: Add stop solution to terminate the reaction. The color changes from blue to yellow immediately. |
| 6 | Reading: Measure absorbance at 450 nm using a microplate reader. |
| Sample Type | Protocol |
| Serum | Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant and store appropriately. |
| Plasma | Collect using EDTA anticoagulant, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma. |
| Cell Culture Supernatant | Centrifuge at 1000 × g for 20 minutes at 4°C and collect clarified supernatant. |
| Cell Lysate | Lyse cells using recommended lysis buffer with protease inhibitors, centrifuge at 10,000 rpm for 10 minutes, and collect protein supernatant. |
| Tissue Homogenate | Homogenize tissue in PBS with protease inhibitors, centrifuge at 5000 × g for 5 minutes, and collect supernatant. |
| Other Sample Types | Centrifuge samples at 1000 × g for 15 minutes at 2–8°C and collect supernatant. For additional guidance, please contact techsupport@assaygenie.com. |
| Component | Quantity | Storage | |
| 48T | 96T | ||
| ELISA Microplate (Dismountable) | 8×6 | 8×12 | Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C. |
| Lyophilized Standard | 1 vial | 2 vial | Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C. |
| Biotin-labeled Antibody (Concentrated, 100X) | 60 ul | 120 ul | 2-8°C (Avoid direct light) |
| HRP-Streptavidin Conjugate (SABC, 100X) | 60 ul | 120 ul | 2-8°C (Avoid direct light) |
| TMB Substrate | 5 ml | 10 ml | 2-8°C (Avoid direct light) |
| Sample Dilution Buffer | 10 ml | 20 ml | 2-8°C |
| Antibody Dilution Buffer | 5 ml | 10 ml | 2-8°C |
| SABC Dilution Buffer | 5 ml | 10 ml | 2-8°C |
| Stop Solution | 5 ml | 5 ml | 2-8°C |
| Wash Buffer(25X) | 15 ml | 30 ml | 2-8°C |
| Plate Sealer | 3 pieces | 5 pieces | - |
| Technical Manual | 1 copy | 1 copy | - |
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