The Porcine Casp9 (Caspase-9) ELISA Kit is specifically designed for the accurate quantification of caspase-9 levels in porcine serum, plasma, and cell culture supernatants. This kit provides high sensitivity and specificity, ensuring dependable and consistent results for various research applications. Caspase-9 is a key enzyme involved in apoptosis, a process of programmed cell death essential for maintaining tissue homeostasis and regulating immune responses. Dysregulation of caspase-9 activity has been implicated in various diseases such as cancer, neurodegenerative disorders, and autoimmune conditions, highlighting the importance of studying its levels as a potential biomarker.
With the Porcine Casp9 ELISA Kit, researchers can accurately measure caspase-9 levels in porcine samples to better understand its role in disease pathogenesis and potentially identify new therapeutic targets. This kit offers a reliable tool for studying apoptosis-related processes in porcine models, aiding in the advancement of biomedical research and drug development efforts.
Product Name:
Porcine Casp9 (Caspase 9) ELISA Kit
SKU:
PRFI00186
Reactivity:
Porcine
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
0.469 ng/mL
Range:
0.781-50 ng/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell Lysis Buffer or Tissue Lysis Buffer, and Other Biological Fluid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
82-98%
87-95%
81-98%
EDTA Plasma (n = 5)
93-100%
80-94%
81-99%
Heparin Plasma (n = 5)
83-97%
82-100%
82-99%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
86-104
96
EDTA Plasma (n = 5)
94-100
93
Heparin Plasma (n = 5)
85-105
96
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
