Phospho-Src (Ser75) In-Cell ELISA

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SKU:
CBCAB01657
Product Type:
ELISA Kit
ELISA Type:
Cell Based Phospho Specific
Research Area:
Cell Biology
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Detection Method:
Colorimetric

Description

Technical Manual

Phospho-Src (Ser75) Cell-Based ELISA

Product Description:The Phospho-Src (Ser75) Colorimetric Cell-Based ELISA Kit allows for qualitative detection of Phospho-Src(Ser75) in plated and fixed cells.
Target Synonyms:c-Src; C-SRC; kinase Src; Neuronal SRC; p60-SRC; p60-Src; Src; SRC; SRC1
Detection Target:Phospho-IRAK1(Thr209)
Reactivity:H:S75, M:S74, R:S75
Pack Size:2 x 96 assays
Detection Method:Colorimetric 450 nm
Format:Cell-Based ELISA/In-Cell ELISA
Storage and Stability Guarantee:4 °C/6 Months
Sample Type:Plated & fixed cells
Range:5000 cells/well minimum
Time:4.5 hours

Phospho-Src (Ser75) Cell-Based ELISA Protein Information

UniProt Protein Function:Src: proto-oncogenic cytoplasmic tyrosine kinase of the SRC family. Highly expressed in certain fully differentiated cells such as neurons, platelets and macrophages. Phosphorylation of an activation loop tyrosine activates the enzyme; phosphorylation of a tyrosine in the C-terminus by Csk inhibits the enzyme. Two alternatively spliced isoforms have been described.
UniProt Protein Details:

Protein type:Oncoprotein; Protein kinase, tyrosine (non-receptor); Kinase, protein; EC 2.7.10.2; Protein kinase, TK; TK group; Src family

Chromosomal Location of Human Ortholog: 20q12-q13

Cellular Component: neuron projection; mitochondrion; lysosome; postsynaptic density; actin filament; caveola; cytosol; extrinsic to internal side of plasma membrane; perinuclear region of cytoplasm; late endosome; mitochondrial inner membrane; cytoplasm; plasma membrane; nucleus

Molecular Function:protein C-terminus binding; ephrin receptor binding; non-membrane spanning protein tyrosine kinase activity; phosphoprotein binding; insulin receptor binding; protein kinase activity; integrin binding; protein binding; enzyme binding; SH3/SH2 adaptor activity; protein kinase C binding; protein-tyrosine kinase activity; heme binding; estrogen receptor binding; SH2 domain binding; kinase activity; ATP binding; receptor binding; hormone receptor binding

Biological Process: oogenesis; regulation of estrogen receptor signaling pathway; estrogen receptor signaling pathway; positive regulation of cyclin-dependent protein kinase activity; central nervous system development; progesterone receptor signaling pathway; nerve growth factor receptor signaling pathway; viral reproduction; regulation of cell cycle; positive regulation of apoptosis; positive regulation of transcription, DNA-dependent; uterus development; negative regulation of mitochondrial depolarization; negative regulation of protein homooligomerization; positive regulation of MAP kinase activity; cell-cell adhesion; transforming growth factor beta receptor signaling pathway; response to electrical stimulus; cell adhesion; bone resorption; response to drug; platelet activation; fibroblast growth factor receptor signaling pathway; activation of protein kinase B; response to virus; transcytosis; positive regulation of integrin activation; positive regulation of protein amino acid autophosphorylation; cellular response to insulin stimulus; response to mechanical stimulus; T cell costimulation; regulation of vascular permeability; negative regulation of transcription, DNA-dependent; leukocyte migration; negative regulation of apoptosis; axon guidance; peptidyl-tyrosine phosphorylation; protein amino acid autophosphorylation; platelet-derived growth factor receptor signaling pathway; negative regulation of caspase activity; signal transduction; positive regulation of smooth muscle cell migration; regulation of cell-cell adhesion; forebrain development; ephrin receptor signaling pathway; epidermal growth factor receptor signaling pathway; integrin-mediated signaling pathway; response to nutrient levels; regulation of bone resorption; negative regulation of focal adhesion formation; signal complex assembly; positive regulation of phosphoinositide 3-kinase activity; response to mineralocorticoid stimulus; cell cycle; regulation of cell proliferation; cell proliferation; positive regulation of protein kinase B signaling cascade; peptidyl-serine phosphorylation; response to hydrogen peroxide; regulation of protein binding; Ras protein signal transduction; stress fiber formation; innate immune response; response to acidity; positive regulation of insulin receptor signaling pathway; vascular endothelial growth factor receptor signaling pathway; blood coagulation; positive regulation of cytokine secretion

NCBI Summary:This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:P12931
NCBI GenInfo Identifier:125711
NCBI Gene ID:6714
NCBI Accession:P12931.3
UniProt Secondary Accession:P12931,Q76P87, Q86VB9, Q9H5A8, E1P5V4,
UniProt Related Accession:P12931
Molecular Weight:60kDa
NCBI Full Name:Proto-oncogene tyrosine-protein kinase Src
NCBI Synonym Full Names:SRC proto-oncogene, non-receptor tyrosine kinase
NCBI Official Symbol:SRC
NCBI Official Synonym Symbols:ASV; SRC1; c-SRC; p60-Src
NCBI Protein Information:proto-oncogene tyrosine-protein kinase Src; proto-oncogene c-Src; tyrosine kinase pp60c-src; tyrosine-protein kinase SRC-1; protooncogene SRC, Rous sarcoma; v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog
UniProt Protein Name:Proto-oncogene tyrosine-protein kinase Src
UniProt Synonym Protein Names:Proto-oncogene c-Src; pp60c-src; p60-Src
Protein Family:Proto-oncogene tyrosine-protein kinase
UniProt Gene Name:SRC
UniProt Entry Name:SRC_HUMAN
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